Tubular epithelial cell-to-macrophage communication forms a negative feedback loop via extracellular vesicle transfer to promote renal inflammation and apoptosis in diabetic nephropathy
| Autor: | Dan-dan Zang, Xiao-Ming Meng, Taotao Ma, Rui Feng, Wenjuan Jiang, Chuanting Xu, Chang-lin Du, Songbing Xu, Cheng Huang, Jun Li, Bing-feng Hu, Jia-hui Dong |
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| Rok vydání: | 2022 |
| Předmět: |
Medicine (miscellaneous)
Apoptosis Cell Communication tumor necrosis factor α Cell Line Diabetic nephropathy Mice Negative feedback Diabetes Mellitus medicine Animals Macrophage Diabetic Nephropathies death receptor 5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) interleukin Chemistry Macrophages diabetic nephropathy tumor necrosis factor-related apoptosis-inducing ligand Epithelial Cells Extracellular vesicle Renal inflammation medicine.disease Epithelium Cell biology Mice Inbred C57BL medicine.anatomical_structure leucine-rich α-2-glycoprotein 1 extracellular vesicles Research Paper |
| Zdroj: | Theranostics |
| ISSN: | 1838-7640 |
| Popis: | Background: Macrophage infiltration around lipotoxic tubular epithelial cells (TECs) is a hallmark of diabetic nephropathy (DN). However, how these two types of cells communicate remains obscure. We previously demonstrated that LRG1 was elevated in the process of kidney injury. Here, we demonstrated that macrophage-derived, LRG1-enriched extracellular vesicles (EVs) exacerbated DN. Methods: We induced an experimental T2DM mouse model with a HFD diet for four months. Renal primary epithelial cells and macrophage-derived EVs were isolated from T2D mice by differential ultracentrifugation. To investigate whether lipotoxic TEC-derived EV (EVe) activate macrophages, mouse bone marrow-derived macrophages (BMDMs) were incubated with EVe. To investigate whether activated macrophage-derived EVs (EVm) induce lipotoxic TEC apoptosis, EVm were cocultured with primary renal tubular epithelial cells. Subsequently, we evaluated the effect of LRG1 in EVe by investigating the apoptosis mechanism. Results: We demonstrated that incubation of primary TECs of DN or HK-2 mTECs with lysophosphatidyl choline (LPC) increased the release of EVe. Interestingly, TEC-derived EVe activated an inflammatory phenotype in macrophages and induced the release of macrophage-derived EVm. Furthermore, EVm could induce apoptosis in TECs injured by LPC. Importantly, we found that leucine-rich α-2-glycoprotein 1 (LRG1)-enriched EVe activated macrophages via a TGFβR1-dependent process and that tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-enriched EVm induced apoptosis in injured TECs via a death receptor 5 (DR5)-dependent process. Conclusion: Our findings indicated a novel cell communication mechanism between tubular epithelial cells and macrophages in DN, which could be a potential therapeutic target. |
| Databáze: | OpenAIRE |
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