Penetapan kadar gentamisin dalam sediaan krim dengan kromatografi lapis tipis - densitometri
| Autor: | Isnaeni Yudi Haryanto, Achmad Burhanudin, Achmad Toto Poernomo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Cultural Studies
Linguistics and Language History gentamicin TLC-densitometry Language and Linguistics Dosage form cream 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Column chromatography Pharmacy and materia medica Cream Dosage Form medicine 030212 general & internal medicine Detection limit Chromatography Spots Silica gel Microbiological assay method validation RS1-441 chemistry 030220 oncology & carcinogenesis Anthropology Gentamicin medicine.drug |
| Zdroj: | Pharmaciana, Vol 6, Iss 2, Pp 101-116 (2016) |
| ISSN: | 2477-0256 2088-4559 |
| Popis: | The used of Gentamicin is still regarded for overcoming infectious diseases. Several preparations of the gentamicin are available in the market as injection, eye or ear drops, and topical dosage forms. Based on the standard method, determination of the gentamicin is carried out by microbiological assay. Several instrumental method have been reported. In this research, Thin Layer Chromatography-Densitometry (TLC-Densitometry) has been validated and used for determining gentamicin in cream dosage form. The TLC was carried out on a Silica gel GF254 using KH2PO4 20% and ninhidrin 2% solution (in ethanol 96%) as eluent and spots visualization respectively. Three spots appeared on the chromatogram having Rf value 0.51, 0.47, and 0.36. All the spots gave maximum absorption at 400 nm. The spot with Rf value of 0.51 was the highest intensity. Limit of Detection (LoD) and Limit of quantitation (LoQ) of the major component was 0.019 and 0.064 respectively. The other validation characteristics met the requirement for determination of gentamicin in the cream dosage form. |
| Databáze: | OpenAIRE |
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