The Nucleoskeleton: A Permanent Structure of Cell Nuclei Regardless of Their Transcriptional Activity
| Autor: | Pavel Hozák, Vlada V. Philimonenko, J.-E. Fléchon |
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| Rok vydání: | 2001 |
| Předmět: |
Amanitins
Transcription Genetic Cell Biology Embryonic and Fetal Development Mice Transcription (biology) medicine Animals Humans Nucleic Acid Synthesis Inhibitors Cell Nucleus Genetics Transcriptional activity RNA Polymerase III Cell Biology Nuclear matrix Cell biology Mice Inbred C57BL Cell nucleus medicine.anatomical_structure Mice Inbred DBA Dactinomycin Ultrastructure Nuclear lamina Female Interphase RNA Polymerase II HeLa Cells |
| Zdroj: | Experimental Cell Research. 264:201-210 |
| ISSN: | 0014-4827 |
| DOI: | 10.1006/excr.2001.5150 |
| Popis: | Nuclear matrix or nucleoskeleton is thought to provide structural basis for intranuclear order. However, the nature of this structure is still uncertain because of numerous technical difficulties in its visualization. To reveal the “real” morphology of the nucleoskeleton, and to identify possible sources of structural artifacts, three methods of nucleoskeleton preparations were compared. The nucleoskeleton visualized by all these techniques consists of identical elements: nuclear lamina and an inner network comprising core filaments and the “diffuse” nucleoskeleton. We then tested if the nucleoskeleton is a stable structure or a transient transcription-dependent structure. Incubation with transcription inhibitors (α-amanitin, actinomycin D, and DRB) for various periods of time had no obvious effect on the morphology of the nucleoskeleton. A typical nucleoskeleton structure was observed also in a physiological model—in transcriptionally inactive mouse 2-cell embryos and in active 8- to 16-cell embryos. Our data suggest that the nucleoskeleton is a permanent structure of the cell nucleus regardless of the nuclear transcriptional state, and the principal architecture of the nucleoskeleton is identical throughout the interphase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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