Investigation of simian virus 40 (SV40) and human JC, BK, MC, KI, and WU polyomaviruses in glioma
| Autor: | Boulbeba Selmi, H. Krifa, Moncef Mokni, Nabiha Missaoui, M. T. Yacoubi, Sarra Limam, Ahlem Bdioui |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male viruses In situ hybridization Simian virus 40 Biology Simian Genome DNA sequencing Virus 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Virology Glioma medicine Humans In Situ Hybridization Polyomavirus Infections Brain Neoplasms Age Factors Middle Aged Viral Load medicine.disease biology.organism_classification Immunohistochemistry JC Virus Survival Analysis 030104 developmental biology Neurology chemistry Merkel cell polyomavirus DNA Viral Capsid Proteins Female Neurology (clinical) Neoplasm Grading Neoplasm Recurrence Local 030217 neurology & neurosurgery DNA Follow-Up Studies |
| Zdroj: | Journal of neurovirology. 26(3) |
| ISSN: | 1538-2443 |
| Popis: | The gliomagenesis remains not fully established and their etiological factors still remain obscure. Polyomaviruses were detected and involved in several human tumors. Their potential implication in gliomas has been not yet surveyed in Africa and Arab World. Herein, we investigated the prevalence of six polyomaviruses (SV40, JCPyV, BKPyV, MCPyV, KIPyV, and WUPyV) in 112 gliomas from Tunisian patients. The DNA sequences of polyomaviruses were examined by PCR assays. Viral infection was confirmed by DNA in situ hybridization (ISH) and/or immunohistochemistry (IHC). The relationships between polyomavirus infection and tumor features were evaluated. Specific SV40 Tag, viral regulatory, and VP1 regions were identified in 12 GBM (10.7%). DNA ISH targeting the whole SV40 genome and SV40 Tag IHC confirmed the PCR findings. Five gliomas yielded JCPyV positivity by PCR and DNA ISH (2.7%). However, no BKPyV, KIPyV, and WUPyV DNA sequences were identified in all samples. MCPyV DNA was identified in 30 gliomas (26.8%). For GBM samples, MCPyV was significantly related to patient age (p = 0.037), tumor recurrence (p = 0.024), and SV40 (p = 0.045) infection. No further significant association was identified with the remaining tumor features (p > 0.05) and patient survival (Log Rank, p > 0.05). Our study indicates the presence of SV40, JCPyV, and MCPyV DNA in Tunisian gliomas. Further investigations are required to more elucidate the potential involvement of polyomaviruses in these destructive malignancies. |
| Databáze: | OpenAIRE |
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