Evidence for a common progenitor of epithelial and mesenchymal components of the liver
| Autor: | M De Santis Puzzonia, Tatiana Kisseleva, Carla Cicchini, Marco Tripodi, P Bianco, Alice Conigliaro, Benedetto Sacchetti, David A. Brenner, Viviana Costa, Carmine Mancone, F Garibaldi, Laura Amicone |
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| Přispěvatelé: | Conigliaro, A., Amicone, L., Costa, V., De Santis Puzzonia, M., Mancone, C., Sacchetti, B., Cicchini, C., Garibaldi, F., Brenner, D., Kisseleva, T., Bianco, P., Tripodi, M., Department of Cellular Biotechnologies and Haematology, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome]-Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), National Institute for Infectious Diseases L. Spallanzani, Istituto di Ricovero e Cura a Carattere Scientifico, Department of Molecular Medicine, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Department of Medicine, University of California [San Diego] (UC San Diego), University of California-University of California, This work was supported by the Italian CancerResearch Foundation, Ministry of Health, The Ministry of University and Scientific Research and the European Molecular Biology Organization. We especially thank Patrizia Lavia and Giulia Guarguaglini (CNR, Rome) for their help in live imagingexperiments |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cellular differentiation
Liver Stem Cell Desmin Mice 0302 clinical medicine MESH: Animals MESH: Nerve Tissue Proteins Hepatic stellate cell Cells Cultured 0303 health sciences Mesenchymal Stromal Cell Stem Cells Cell Differentiation Cell biology Endothelial stem cell MESH: Desmin MESH: Models Animal Liver MESH: Epithelial Cells Differentiation Models Animal 030211 gastroenterology & hepatology Stem cell MESH: Stem Cell Transplantation hepatic stellate cell cell transplantation liver stem cell differentiation MESH: Cells Cultured MESH: Cell Differentiation Cell transplantation Liver stem cell Animals Cell Line Cell Lineage Cell Proliferation Epithelial Cells Glial Fibrillary Acidic Protein In Vitro Techniques Mesenchymal Stromal Cells Mice Nude Nerve Tissue Proteins Stem Cell Transplantation Cell Biology Molecular Biology Clinical uses of mesenchymal stem cells MESH: Stem Cells [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology 03 medical and health sciences Stem Cell MESH: Cell Proliferation MESH: Mice Nude Progenitor cell MESH: Mice 030304 developmental biology Original Paper Epithelial Cell Animal In Vitro Technique Mesenchymal stem cell Mesenchymal Stem Cells MESH: Cell Lineage MESH: Cell Line Nerve Tissue Protein MESH: Mesenchymal Stromal Cells MESH: Liver |
| Zdroj: | Cell Death and Differentiation Cell Death and Differentiation, Nature Publishing Group, 2013, 20 (8), pp.1116-23. ⟨10.1038/cdd.2013.49⟩ |
| ISSN: | 1350-9047 1476-5403 |
| DOI: | 10.1038/cdd.2013.49⟩ |
| Popis: | Tissues of the adult organism maintain the homeostasis and respond to injury by means of progenitor/stem cell compartments capable to give rise to appropriate progeny. In organs composed by histotypes of different embryological origins (e.g. The liver), the tissue turnover may in theory involve different stem/precursor cells able to respond coordinately to physiological or pathological stimuli. In the liver, a progenitor cell compartment, giving rise to hepatocytes and cholangiocytes, can be activated by chronic injury inhibiting hepatocyte proliferation. The precursor compartment guaranteeing turnover of hepatic stellate cells (HSCs) (perisinusoidal cells implicated with the origin of the liver fibrosis) in adult organ is yet unveiled. We show here that epithelial and mesenchymal liver cells (hepatocytes and HSCs) may arise from a common progenitor. Sca+ murine progenitor cells were found to coexpress markers of epithelial and mesenchymal lineages and to give rise, within few generations, to cells that segregate the lineage-specific markers into two distinct subpopulations. Notably, these progenitor cells, clonally derived, when transplanted in healthy livers, were found to generate epithelial and mesenchymal liver-specific derivatives (i.e. hepatocytes and HSCs) properly integrated in the liver architecture. These evidences suggest the existence of a 'bona fide' organ-specific meso-endodermal precursor cell, thus profoundly modifying current models of adult progenitor commitment believed, so far, to be lineage-restricted. Heterotopic transplantations, which confirm the dual differentiation potentiality of those cells, indicates as tissue local cues are necessary to drive a full hepatic differentiation. These data provide first evidences for an adult stem/precursor cell capable to differentiate in both parenchymal and non-parenchymal organ-specific components and candidate the liver as the instructive site for the reservoir compartment of HSC precursors as yet non-localized in the adult. © 2013 Macmillan Publishers Limited All rights reserved. |
| Databáze: | OpenAIRE |
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