Local autoimmune encephalomyelitis model in a rat brain with precise control over lesion placement
| Autor: | Lukasz Kalkowski, Dominika Golubczyk, Joanna Kwiatkowska, Malgorzata Domzalska, Piotr Walczak, Izabela Malysz-Cymborska |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Central Nervous System Encephalomyelitis Autoimmune Experimental Multiple Sclerosis Histology Science Immune Cells Immunology Nervous System Antibodies Autoimmune Diseases White Blood Cells Medical Conditions Signs and Symptoms Cell Signaling Antigen Encapsulation Animal Cells Medicine and Health Sciences Animals Rats Wistar Drug Delivery System Preparation Leukocyte Signaling Injections Intraventricular Multidisciplinary Blood Cells Pharmaceutics Pharmaceutical Processing Technology Brain Biology and Life Sciences Neurodegenerative Diseases Cell Biology Demyelinating Disorders Rats Neuroanatomy Spinal Cord Neurology Lesions Medicine Cattle Clinical Immunology Clinical Medicine Cellular Types Anatomy Research Article Neuroscience Signal Transduction |
| Zdroj: | PLoS ONE PLoS ONE, Vol 17, Iss 1 (2022) PLoS ONE, Vol 17, Iss 1, p e0262677 (2022) |
| ISSN: | 1932-6203 |
| Popis: | Development of a novel, animal model for multiple sclerosis (MS) with reproducible and predictable lesion placement would enhance the discovery of effective treatments. Therefore, we would like to combine the advantages of the demyelination model with experimental autoimmune encephalomyelitis (EAE) to provide a local autoimmune encephalomyelitis (LAE) inside rat brain. We induced a demyelinating lesion by immunizing male Wistar rats, followed by blood-brain barrier opening protein (vascular endothelial growth factor) by stereotactic injection. We confirmed the immunization against myelin epitopes and minor neurological impairment. Histological assessment confirmed the lesion development after both 3- and 7 days post-injection. Our approach was sufficient to develop a demyelinating lesion with high reproducibility and low morbidity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|