Polyphenols-enriched Hibiscus sabdariffa extract-loaded nanostructured lipid carriers (NLC): Optimization by multi-response surface methodology
| Autor: | Eliana B. Souto, Antonio Segura-Carretero, David Arráez-Román, S. Pimentel-Moral, Armando Fernandes, Mônica Cristina Teixeira, Antonio Martínez-Férez, Isabel Borrás-Linares |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Ciências Agrárias::Biotecnologia Agrária e Alimentar
Biotecnologia Agrária e Alimentar [Ciências Agrárias] Engenharia e Tecnologia::Biotecnologia Industrial Pharmaceutical Science 02 engineering and technology 030226 pharmacology & pharmacy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pulmonary surfactant Response surface methodology Biotecnologia Industrial [Engenharia e Tecnologia] Zeta potential Hibiscus sabdariffa Chromatography Science & Technology Nanostructured lipid Carrier Polyphenols 021001 nanoscience & nanotechnology Bioavailability chemistry Polyphenol Nanoparticles Encapsulation Particle size 0210 nano-technology Quercetin |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Nanoparticles are commonly used to improve the bioavailability of several bioactive compounds. In this work, nanostructured lipid carriers (NLC) have been optimized by response-surface methodology to protect phenolic compounds from Hibiscus sabdariffa. The influence of lipid and surfactant concentration on the mean particle size, polydispersity index and zeta potential of NLC was analysed. The results showed that the effect of both independent variables was statistically significantly on the selected responses. The optimized formulation obtained from the predicted model was composed of 2.21% (w/w) of lipid phase and 1.93% (w/w) of surfactant, with a desirability function of 0.77. The loading of polyphenol-enriched Hibiscus sabdariffa extract - obtained by microwave assisted extraction or by pressurized liquid extraction - within the NLC resulted in particles with a mean size of 470±8nm (encapsulation efficiency of 52.9±0.9% for quercetin and 60±2% for anthocyanins) and 344±12nm (encapsulation efficiency of 93±3% for quercetin and 84±4% for anthocyanins), respectively. Transmission electron microscopy images and Fourier transformed infra-red analysis of extracts-loaded NLC confirmed that interaction between lipids and polyphenols improved the physicochemical stability of the particles. Our findings highlight the added value of NLC as a delivery system for phenolic compounds extracted from Hibiscus sabdariffa. Research group AGR274 “Bioactive ingredients” from the Analytical Chemistry Department of the University of Granada, and the Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra. Also, the authors would like to thank the financial support received from the Spanish Ministry of Economy and Competitiveness (MINECO) (project AGL2015-67995-C3-2), from Portuguese Science and Technology Foundation (FCT/MCT) and from European Funds (PRODER/COMPETE) under the project M-ERA-NET/0004/2015-PAIRED, co-financed by FEDER, under the Partnership Agreement PT2020. We would like to thank the Ministry of Education, Culture and Sport (MECD) for supporting the grant FPU15/01125 of SPM and the stay in the University of Coimbra supported by FPU short – term stay. MCT wishes to acknowledge FCT and Dendropharma - Investigação E Serviços De Intervenção Farmacêutica, Sociedade Unipessoal Lda. for the individual fellowship (PD/BDE/135086/2017) info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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