Increase in Salivary Cysteinyl-Leukotriene Concentration in Patients with Aspirin-Intolerant Asthma
| Autor: | Mamoru Otomo, Maki Hasegawa, Naomi Tsurikisawa, Noritaka Higashi, Yuma Fukutomi, Haruhisa Mita, Chiyako Oshikata, Kazuo Akiyama, Hidenori Tanimoto, Takahiro Tsuburai, Yuji Maeda, Emiko Ono, Kiyoshi Sekiya, Sayaka Tatsuno, Masami Taniguchi, Toshihide Kumamoto, Hiromichi Yamaguchi, Eishi Miyazaki |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Leukotrienes Saliva Leukotriene B4 Metabolite Urine chemistry.chemical_compound fluids and secretions stomatognathic system medicine Humans Immunology and Allergy Exhaled breath condensate Cysteine Aged Asthma Aspirin medicine.diagnostic_test business.industry Anti-Inflammatory Agents Non-Steroidal General Medicine Middle Aged respiratory system medicine.disease stomatognathic diseases Cross-Sectional Studies chemistry Immunoassay Exhaled nitric oxide Immunology Asthma Aspirin-Induced Female lipids (amino acids peptides and proteins) business |
| Zdroj: | Allergology International. 60:37-43 |
| ISSN: | 1323-8930 |
| DOI: | 10.2332/allergolint.09-oa-0166 |
| Popis: | Background Cysteinyl-leukotrienes (CysLTs; LTC4, LTD4, and LTE4) play a considerable role in the pathophysiology of aspirin-intolerant asthma (AIA). Saliva has recently been validated as novel, simple, and noninvasive method for investigating inflammation in patients with asthma. The aim of this study is to clarify the molecular species of CysLT in saliva and to evaluate the CysLT and LTB4 concentrations in saliva in AIA patients. We also examined how the CysLT concentration in saliva reflects that of their corresponding urinary metabolite. Methods We preformed an analytical cross-sectional study. CysLT and LTB4 concentrations in saliva were quantified by enzyme immunoassay (EIA) following purification by high-performance liquid chromatography (HPLC). Results: 1. When analyzed by EIA in combination with HPLC, saliva was found to consist of LTC4, LTD4 and LTE4 in similar amounts. 2. In saliva analysis among the three groups (AIA patients, aspirin-tolerant asthma [ATA] patients, and healthy subjects), both the concentrations of CysLTs and LTB4 were significantly higher in AIA patients than in ATA patients and healthy subjects. 3. We found significant correlations between CysLT concentration and LTB4 concentration in saliva in each group. 4. No significant correlation was found between the concentration of LTE4 in urine and that of CysLTs in saliva. Conclusions In this study, we found higher concentrations of CysLTs and LTB4 in saliva from AIA patients than in saliva from ATA patients, suggesting that the quantification of CysLT and LTB4 concentrations in saliva may be another diagnostic strategy for AIA. |
| Databáze: | OpenAIRE |
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