G‐Quadruplex Formation in a Putative Coding Region of White Spot Syndrome Virus: Structural and Thermodynamic Aspects
| Autor: | Klaus Weisz, Yoanes Maria Vianney, Maria Goretti Marianti Purwanto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Circular dichroism
Stereochemistry Mutant White spot syndrome Calorimetry 010402 general chemistry G-quadruplex 01 natural sciences Biochemistry white spot syndrome virus Conserved sequence thermodynamics NMR spectroscopy White spot syndrome virus 1 Coding region Molecular Biology Sequence (medicine) biology 010405 organic chemistry Chemistry Communication Organic Chemistry Nuclear magnetic resonance spectroscopy biology.organism_classification Communications 0104 chemical sciences G-Quadruplexes DNA Viral Molecular Medicine Nucleic Acid Conformation |
| Zdroj: | Chembiochem |
| ISSN: | 1439-7633 1439-4227 |
| Popis: | White spot disease (WSD) is one of the most devastating viral infections of crustaceans caused by the white spot syndrome virus (WSSV). A conserved sequence WSSV131 in the DNA genome of WSSV was found to fold into a polymorphic G‐quadruplex structure. Supported by two mutant sequences with single G→T substitutions in the third G4 tract of WSSV131, circular dichroism and NMR spectroscopic analyses demonstrate folding of the wild‐type sequence into a three‐tetrad parallel topology comprising three propeller loops with a major 1 : 3 : 1 and a minor 1 : 2 : 2 loop length arrangement. A thermodynamic analysis of quadruplex formation by differential scanning calorimetry (DSC) indicates a thermodynamically more stable 1 : 3 : 1 loop isomer. DSC also revealed the formation of additional highly stable multimeric species with populations depending on potassium ion concentration. The right spot for white spot: A G‐quadruplex‐forming sequence identified within the DNA genome of white spot syndrome virus folds into a major and a thermodynamically less stable minor loop isomer. The parallel quadruplex scaffold might constitute a potential target for viral drug development against white spot disease. |
| Databáze: | OpenAIRE |
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