Development and automation of a novel NET-PET tracer: [11C]Me@APPI
| Autor: | Matthias Hendl, Lukas Nics, Birgit Bornatowicz, Wolfgang Wadsak, Daniela Haeusler, Rupert Lanzenberger, Markus Mitterhauser, Christina Mark, Michael L. Berger, Helmut Spreitzer |
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| Rok vydání: | 2012 |
| Předmět: |
Quality Control
Cancer Research Analytical chemistry Automation Drug Stability Humans Radiology Nuclear Medicine and imaging Carbon Radioisotopes Chromatography Norepinephrine Plasma Membrane Transport Proteins Radiochemistry biology Chemistry Radiosynthesis Transporter Membrane Norepinephrine transporter Blood-Brain Barrier Yield (chemistry) Isotope Labeling Positron-Emission Tomography Microsome biology.protein Molecular Medicine Specific activity Benzimidazoles Selectivity |
| Zdroj: | Nuclear medicine and biology. 40(2) |
| ISSN: | 1872-9614 |
| Popis: | Introduction The norepinephrine transporter (NET) is an important target for research in neurology and psychology and is involved in the pathophysiology of many neurodegenerative diseases such as Alzheimer's disease and attention deficient hyperactivity disorder. For visualization of NET abundance and deregulation, a novel PET tracer – [ 11 C]Me@APPI – has been developed. Methods For precursor synthesis, a 4-step synthesis starting from N-phenyl-o-phenylenediamine was set up. Radiosynthesis was established and optimized using standard methods and subsequently automated in a GE TRACERlabFx C Pro synthesizer. Preclinical testing was performed comprising affinity and selectivity testing on human membranes as well as stability and blood–brain-barrier-penetration using in-vitro models. Results Precursor molecule (APPI:0) and reference compound (Me@APPI) were synthesized with 26.5% and 21.4% overall yield, respectively. So far, 1.25±0.72GBq [ 11 C]Me@APPI with 54.35±7.80GBq/μmol specific activity were produced (n=11). Affinity of reference compounds was determined as 8.08±1.75 nM for Me@APPI and 19.31±2.91 nM for APPI:0, respectively (n≥9). IAM-chromatography experiments (n=3) revealed a P m value of 1.51±0.34 for Me@APPI. Stability testing using human liver microsomes revealed that 99.5% of the tracer was found to be still intact after 60minutes (n=4). Conclusion Present data indicate that [ 11 C]Me@APPI has promising properties to become a clinically useful NET-PET-tracer. Further in-vitro and in-vivo evaluations are currently under way. |
| Databáze: | OpenAIRE |
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