Genomic sequencing of two isolates of Ralstonia solanacearum causing Sergipe facies and comparative analysis with Bugtok disease isolates
| Autor: | Greecy M. R. Albuquerque, Elineide Barbosa de Souza, Ana Karolina Leite Pais, Valdir de Queiroz Balbino, Wilson José da Silva Junior, Adriano Márcio Freire Silva, Marco Aurélio Siqueira da Gama, Rosa de Lima Ramos Mariano, Jessica Rodrigues da Silva, Maria Esther de N. Fonseca |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Phylotype Genetics Genetics of Microorganisms Ralstonia solanacearum Contig Genomic sequencing Disease Biology QH426-470 biology.organism_classification 01 natural sciences Genome 03 medical and health sciences 030104 developmental biology Facies Banana tree phylotype Molecular Biology Gene inflorescence infection 010606 plant biology & botany |
| Zdroj: | Genetics and Molecular Biology, Volume: 43, Issue: 4, Article number: e20200155, Published: 06 NOV 2020 Genetics and Molecular Biology, Vol 43, Iss 4 (2020) Genetics and Molecular Biology v.43 n.4 2020 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG |
| Popis: | Ralstonia solanacearum is the causal agent of Moko disease in bananas, which in the state of Sergipe in northeastern Brazil causes “Sergipe facies”. This disease induces atypical symptoms similar to those of Bugtok disease in the Philippines. This study was conducted to sequence, assemble, and annotate the genomes of the Sergipe facies-causing isolates SFC and IBSBF2570 (sequevar IIA-53) and compare their genomes with two representative isolates causing Bugtok disease. The genomes were sequenced and assembled, resulting in lengths of 5.58 Mb (SFC) and 5.46 Mb (IBSBF2570) in 185 and 174 contigs, respectively. The isolates of Sergipe facies and Bugtok disease showed similarities in their gene contents. We identified 5,668 information clusters, 3,752 of which were shared by all genomes (core genes). Moreover, 3,585 single-copy genes were identified. Isolates causing Bugtok disease exclusively shared 266 more information clusters than the isolates causing Sergipe facies. These results suggest that Sergipe facies and Bugtok disease isolates show high genomic similarity. However, the similarity is even greater between the Bugtok disease isolates. This may be because of their longer period of interaction compared to Sergipe facies isolates. |
| Databáze: | OpenAIRE |
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