Growth arrest of a murine mesangial cell line by transforming growth factor beta 1 is associated with inhibition of mitogen-induced Ca2+ mobilization
| Autor: | Wenyao Shi, Gyorgy Baffy, Kumar Sharma, John R. Williamson, Fuad N. Ziyadeh |
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| Rok vydání: | 1995 |
| Předmět: |
Indoles
medicine.medical_treatment Biophysics Biochemistry AKT3 Cell Line Maleimides Mice Growth factor receptor Transforming Growth Factor beta medicine Animals Growth factor receptor inhibitor Molecular Biology Protein kinase C Protein Kinase C Platelet-Derived Growth Factor Mesangial cell biology Growth factor Cell Biology Cell biology Glomerular Mesangium biology.protein Calcium Mitogens Platelet-derived growth factor receptor Transforming growth factor |
| Zdroj: | Biochemical and biophysical research communications. 210(2) |
| ISSN: | 0006-291X |
| Popis: | Transforming growth factor-beta (TGF beta) is a multifunctional cytokine showing growth effects on many cell types. In the present study effects of TGF beta 1 on mitogen-induced Ca2+ responses were investigated in an immortalized murine mesangial cell line where TGF beta 1 effects on growth are inhibitory. TGF beta 1 was found to inhibit intracellular Ca2+ mobilization induced by platelet-derived growth factor (PDGF). This effect was completely reversed by previous addition of the non-specific serine/threonine kinase inhibitor H-7, but was unaffected by GF 109203X, a specific inhibitor of protein kinase C (PKC). These findings suggest that inhibition of mitogen-induced Ca2+ mobilization by TGF beta 1 appears not to involve prior activation of PKC, but may participate in the mechanisms whereby mesangial cell growth is inhibited by TGF beta 1. |
| Databáze: | OpenAIRE |
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