Suppressing active replication of a live attenuated simian immunodeficiency virus vaccine does not abrogate protection from challenge
| Autor: | Roland Plesker, Norbert Bannert, Oliver Hohn, Klaus Cichutek, Benjamin Gabriel, Stephen Norley, Cheick Coulibaly, Reinhard Kurth, Uwe Fiebig, Michael D. Mühlebach |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Protective immunity T-Lymphocytes animal diseases viruses Simian Acquired Immunodeficiency Syndrome Viremia Antibodies Viral Vaccines Attenuated Virus Replication medicine.disease_cause Macaque 03 medical and health sciences Animal model Immune system Virology biology.animal medicine Animals AIDS Vaccines B-Lymphocytes biology virus diseases Simian immunodeficiency virus medicine.disease Macaca mulatta 030104 developmental biology Viral replication Immunology Simian Immunodeficiency Virus |
| Zdroj: | Virology. 489:1-11 |
| ISSN: | 0042-6822 |
| Popis: | Although safety concerns preclude the use of live attenuated HIV vaccines in humans, they provide a useful system for identifying the elusive correlates of protective immunity in the SIV/macaque animal model. However, a number of pieces of evidence suggest that protection may result from prior occupancy of susceptible target cells by the vaccine virus rather than the immune response. To address this, we developed a Nef-deletion variant of an RT-SHIV whose active replication could be shut off by treatment with RT-inhibitors. Groups of macaques were inoculated with the ∆Nef-RT-SHIV and immune responses allowed to develop before antiretroviral treatment and subsequent challenge with wild-type SIVmac239. Vaccinated animals either resisted infection fully or significantly controlled the subsequent viremia. However, there was no difference between animals undergoing replication of the vaccine virus and those without. This strongly suggests that competition for available target cells does not play a role in protection. |
| Databáze: | OpenAIRE |
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