The Coli Surface Antigen CS3 of Enterotoxigenic Escherichia coli Is Differentially Regulated by H-NS, CRP, and CpxRA Global Regulators
| Autor: | Javier Torres, Jorge A. Girón, Diana Rodríguez-Valverde, Miguel A. Ares, María Dolores Alcántar-Curiel, María L Cedillo, Miguel Cruz, Judith Abundes-Gallegos, Ma Dolores Jarillo-Quijada, Leonardo G. Panunzi, César Jiménez-Galicia |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
H-NS CpxRA lcsh:QR1-502 Repressor medicine.disease_cause Microbiology lcsh:Microbiology 03 medical and health sciences enterotoxigenic E. coli Transcription (biology) Enterotoxigenic Escherichia coli Gene cluster Gene expression medicine Gene Original Research 030304 developmental biology 0303 health sciences Osmotic concentration biology 030306 microbiology Chemistry Pilin biology.protein CRP CS3 |
| Zdroj: | Frontiers in Microbiology, Vol 10 (2019) Frontiers in Microbiology |
| Popis: | Enterotoxigenic Escherichia coli produces a myriad of adhesive structures collectively named colonization factors (CFs). CS3 is a CF, which is assembled into fine wiry fibrillae encoded by the cstA-H gene cluster. In this work we evaluated the influence of environmental cues such as temperature, osmolarity, pH, and carbon source on the expression of CS3 genes. The transcription of cstH major pilin gene was stimulated by growth of the bacteria in colonization factor broth at 37°C; the presence of glycerol enhanced cstH transcription, while glucose at high concentration, high osmolarity, and the depletion of divalent cations such as calcium and magnesium repressed cstH expression. In addition, we studied the role of H-NS, CpxRA, and CRP global regulators in CS3 gene expression. H-NS and CpxRA acted as repressors and CRP as an activator of cstH expression. Under high osmolarity, H-NS, and CpxRA were required for cstH repression. CS3 was required for both, bacterial adherence to epithelial cells and biofilm formation. Our data strengthens the existence of a multi-factorial regulatory network that controls transcription of CS3 genes in which global regulators, under the influence of environmental signals, control the production of this important intestinal colonization factor. |
| Databáze: | OpenAIRE |
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