Pterostilbene attenuates lipopolysaccharide-induced learning and memory impairment possibly via inhibiting microglia activation and protecting neuronal injury in mice
| Autor: | Lingling Ding, Feng-rong Miao, Chunfu Wu, Yanhua Mou, Guanbo Xie, Lihui Wang, Jingyu Yang, Guangyue Su, Yue Hou, Guoliang Chen |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
Doublecortin Protein Pterostilbene Cell Survival Morris water navigation task Hippocampal formation Pharmacology Hippocampus Cell Line Random Allocation chemistry.chemical_compound Cell Line Tumor Stilbenes medicine Animals Humans Maze Learning Nootropic Agents Biological Psychiatry Neurons Memory Disorders Dose-Response Relationship Drug biology Microglia Learning Disabilities Chemistry Long-term potentiation Doublecortin Disease Models Animal Neuroprotective Agents medicine.anatomical_structure nervous system biology.protein Tumor necrosis factor alpha NeuN Neuroscience |
| Zdroj: | Progress in Neuro-Psychopharmacology and Biological Psychiatry. 54:92-102 |
| ISSN: | 0278-5846 |
| DOI: | 10.1016/j.pnpbp.2014.03.015 |
| Popis: | The present study aims to evaluate the effects of pterostilbene on lipopolysaccharide (LPS)-induced learning and memory impairment as well as the possible changes of microglia and neurons. Firstly, learning and memory function was investigated by behavioral tests. Pterostilbene attenuated LPS-induced learning and memory impairment tested by Y-maze and Morris water maze. Secondly, immunohistochemical method was used to study the changes of microglia and neurons. The results showed that pterostilbene produced a significant decrease in the number of Iba-1 and Doublecortin (DCX) positive cells and a significant increase in neuronal nuclear antigen (NeuN)-stained area of neurons in mouse hippocampal compared to the LPS group. Finally, an in vitro study was performed to further confirm the inhibitory effect on microglia activation and protective effect on neurons exerted by pterostilbene. The results demonstrated that pterostilbene significantly inhibited microglia activation, showing the obvious decrease of LPS-induced production of NO, TNF-α and IL-6 in N9 microglial cells. In addition, the viability of SH-SY5Y cells decreased by conditioned media of LPS-activated N9 microglial cells was remarkably recovered by pterostilbene. In summary, the present study demonstrated for the first time that pterostilbene attenuated LPS-induced learning and memory impairment, which may be associated with its inhibitory effect on microglia activation and protective effect on neuronal injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect