Preservation and reactivation of Candidatus Jettenia asiatica and Anammoxoglobus propionicus using different preservative agents
| Autor: | Airton Kunz, L. A. Scussiato, Marina Celant De Prá, Maurício Egídio Cantão, Aline Viancelli, Adriana Mércia Guaratini Ibelli |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Glycerol Preservative Environmental Engineering Swine Health Toxicology and Mutagenesis Microorganism 030106 microbiology Microbial Consortia Preservation Biological Biomass 010501 environmental sciences Biology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Bioreactors Environmental Chemistry Animals 0105 earth and related environmental sciences Sewage Public Health Environmental and Occupational Health food and beverages General Medicine General Chemistry biology.organism_classification Pollution Manure Culture Media Planctomycetales Milk chemistry Microbial population biology Anammox Cattle Female Oxidation-Reduction Bacteria |
| Zdroj: | Chemosphere. 186 |
| ISSN: | 1879-1298 |
| Popis: | Anaerobic ammonium oxidation (anammox) bacteria have peculiar characteristics that make them difficult to cultivate. The conservation of these microorganisms in culture collections or laboratories requires successful preservation and reactivation techniques. Furthermore, studies have shown that successful reactivation may be preservative dependent. Considering this, the present study aimed to evaluate the preservation and reactivation of anammox consortia enriched from swine manure treatment lagoons, by using different preservative agents at different temperatures: KNO3 (at 4 °C), glycerol (−20 °C, −80 °C), and skimmed cow milk (−20 °C, −80 °C, −200 °C). After 4 months, the biomass was thawed (except for KNO3), and the reestablishment of anammox activity was evaluated by stoichiometric coefficients. Microbial community transformation during the reactivation process was also studied by 16S rDNA sequence analysis. The results showed that the anammox biomass preserved with glycerol or skimmed cow milk at −80 °C recovered activity, while the biomass preserved with other methodologies did not reestablish activity during the studied time (90 days). The bacterial community from the biomass with anammox activity was characterized and showed the presence of Candidatus Brocadia anammoxidans, Candidatus Jettenia asiatica, and Candidatus Anammoxoglobus propionicus. Preservation with skimmed cow milk at −80 °C favored the selection of Candidatus Anammoxoglobus propionicus, while preservation with glycerol at −80 °C was successful for Candidatus Jettenia asiatica. The present study was effective on anammox sludge preservation and reactivation using low-cost processes for anammox cultures preservation, which is important for biomass transport and deammonification reactor start up. |
| Databáze: | OpenAIRE |
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