Determination of Serum 25-Hydroxyvitamin D-3 by LC/MS/MS and Its Monthly Variation in Sapporo Indoor Workers
| Autor: | Maiko Yamamoto, Shu-Ping Hui, Zhen Chen, Hiroaki Okabe, Hideyuki Imai, Hitoshi Chiba, Rei Kikuchi, Akiko Minami, Masahiro Mizuta, Chikara Shimizu, Yifan Chen |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
25-Hydroxy vitamin D-3
Blood Chemical Analysis LC/MS/MS Chemistry Metabolite 010401 analytical chemistry UV-B Chromatography liquid 030209 endocrinology & metabolism Serum samples 01 natural sciences monthly variation 0104 chemical sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Animal science Lc ms ms Vitamin D and neurology Calcifediol |
| Zdroj: | Analytical sciences. 34(9):1043-1047 |
| ISSN: | 0910-6340 |
| Popis: | 25-Hydroxyvitamin D-3 (25(OH)D-3) as the metabolite of vitamin D, is connected with various of diseases, and important to people with limited sunshine. Thus, the investigation of serum 25-hydroxyvitamin D and its variation in these people is necessary. In this study, a simple, precise, and accurate method for serum 25(OH)D-3 determination by LC/MS/MS was developed. Serum samples were obtained monthly for one year from 11 male and 11 female indoor workers in Sapporo, Japan, and the overall 25(OH)D-3 concentration was 12.9 +/- 4.7 ng/mL. The 25(OH)D-3 in females was significantly lower than that in males (14.0 +/- 5.0 vs. 11.9 +/- 4.3 ng/mL). The serum 25(OH)D-3 concentration in males and females were both strongly correlated to UV-B radiation (r(2) = 0.8477 and 0.7384, respectively), with a two-month's lag. Also the monthly change in 25(OH)D-3 in males was more significant than that in females. |
| Databáze: | OpenAIRE |
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