Diurnal Regulation of Plant Epidermal Wax Synthesis through Antagonistic Roles of the Transcription Factors SPL9 and DEWAX
Autor: | Jang Chol Kim, Rong Jun Li, Xiu Lin Liu, Shiyou Lü, Matthew A. Jenks, Lin Mao Li |
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Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Mutant Arabidopsis Plant Science 01 natural sciences Plant Epidermis 03 medical and health sciences Gene Expression Regulation Plant Stress Physiological Gene expression Arabidopsis thaliana Gene Transcription factor Research Articles Regulation of gene expression Wax biology Arabidopsis Proteins Cell Biology biology.organism_classification Plants Genetically Modified Aldehyde Oxidoreductases Cell biology DNA-Binding Proteins MicroRNAs 030104 developmental biology visual_art Waxes visual_art.visual_art_medium Trans-Activators 010606 plant biology & botany Transcription Factors |
Zdroj: | Plant Cell |
ISSN: | 1532-298X |
Popis: | Plant surface waxes form an outer barrier that protects the plant from many forms of environmental stress. The deposition of cuticular waxes on the plant surface is regulated by external environmental changes, including light and dark cycles. However, the underlying molecular mechanisms controlling light regulation of wax production are still poorly understood, especially at the posttranscriptional level. In this paper, we report the regulation of cuticular wax production by the miR156-SPL9 (SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9) module in Arabidopsis (Arabidopsis thaliana). When compared with wild-type plants, miR156 and SPL9 mutants showed significantly altered cuticular wax amounts in both stems and leaves. Furthermore, it was found that SPL9 positively regulates gene expression of the alkane-forming enzyme ECERIFERUM1 (CER1), as well as the primary (1-) alcohol-forming enzyme ECERIFERUM4 (CER4), to enhance alkane and 1-alcohol synthesis, respectively. Our results indicate that complex formation of SPL9 with a negative regulator of wax synthesis, DEWAX, will hamper SPL9 DNA binding ability, possibly by interfering with SPL9 homodimerization. Combined with their diurnal gene and protein expressions, this dynamic repression-activation transcriptional module defines a dynamic mechanism that may allow plants to optimize wax synthesis during daily cycles. These findings provide a regulatory framework for environmental signal integration in the regulation of wax synthesis. |
Databáze: | OpenAIRE |
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