Rosmarinic acid alleviates ethanol-induced lipid accumulation by repressing fatty acid biosynthesis
| Autor: | Jingfan Ma, Yu Shangguan, Guoqing Xue, Longxin Qiu, Yanxin Ruan, Chang Guo, Meiru Zhang, Jian Yang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Depsides Cell Line Palmitic acid Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Stress Physiological Animals Ethanol biology Chemistry Rosmarinic acid Fatty Acids Lipid metabolism General Medicine Metabolism Lipid Metabolism Malondialdehyde Fatty acid synthase 030104 developmental biology Biochemistry Cinnamates Succinic acid 030220 oncology & carcinogenesis Glycine Hepatocytes biology.protein Food Science |
| Zdroj: | Food & Function. 11:2094-2106 |
| ISSN: | 2042-650X 2042-6496 |
| DOI: | 10.1039/c9fo02357g |
| Popis: | Recent studies have demonstrated that rosmarinic acid is a valuable natural product for treatment of alcoholic liver disease. However, the mechanisms whereby rosmarinic acid improves alcoholic liver disease remain unclear. Here we performed experiments using a non-transformed mouse hepatocyte cell line (AML12). Oil-red O staining demonstrated that rosmarinic acid reduced ethanol-induced lipid accumulation. It was shown that rosmarinic acid prevented ethanol-induced elevation of the malondialdehyde level. We also found that rosmarinic acid inhibited ethanol-induced mRNA expression of tumor necrosis factor-α and interleukin 6. Metabolomics analysis revealed that rosmarinic acid ameliorated ethanol-induced fatty acid biosynthesis in the cytoplasm. In addition, palmitic acid was a candidate biomarker in cells exposed to ethanol or ethanol plus rosmarinic acid. Rosmarinic acid prevented the ethanol-induced increase in sorbitol that is a component of the polyol pathway. Moreover, we confirmed that rosmarinic acid attenuated ethanol-induced mRNA expression of fatty acid synthase, probably by modulating the AMPK/SREBP-1c pathway. Furthermore, rosmarinic acid prevented the ethanol-induced decrease in eight metabolites that are involved in mitochondrial metabolism, including glycine and succinic acid which are the components of carnitine synthesis. These results provide a crucial insight into the molecular mechanism of rosmarinic acid in alleviating ethanol-induced injury. |
| Databáze: | OpenAIRE |
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