Novel strategy for quality consistency evaluation of Chinese medicine 'YIQING' tablet that combines the simultaneous quantification and screening of ten bioactive constituents
Autor: | Jing Zhang, Dandan Gong, Yilang Hong, Guoxiang Sun |
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Rok vydání: | 2017 |
Předmět: |
Quality Control
Jatrorrhizine Principal Component Analysis Analyte Chromatography 010405 organic chemistry DPPH 010401 analytical chemistry Filtration and Separation 01 natural sciences 0104 chemical sciences Analytical Chemistry Baicalein Chemometrics chemistry.chemical_compound chemistry Fingerprint Medicine Chinese Traditional Emodin Baicalin Chromatography High Pressure Liquid Drugs Chinese Herbal Tablets |
Zdroj: | Journal of Separation Science. 40:3064-3073 |
ISSN: | 1615-9306 |
Popis: | The UV characteristics for different categories compounds in complex traditional Chinese medicines and herbal preparations usually vary. Thus, to achieve the integral analysis of multiwavelength fingerprint characteristics, we introduced a novel strategy of multiwavelength total fusion profiling. The simultaneous separation and quantification of multiple components by reversed-phase high-performance liquid chromatography coupled with diode array detection was developed in an effective, accurate, and reliable way. Furthermore, a 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging assay was set up to detect and screen the bioactivity of similar-structure constituents (aloe-emodin, rhein, emodin, chrysophanol, baicalein, wogonin, baicalin, wogonoside, berberine hydrochloride, and jatrorrhizine hydrochloride). Moreover, the high-performance liquid chromatography DPPH assay was developed to monitor the relationship between the biological activity and the spatial structure, the number of hydroxyl groups, the concentration of the analytes in samples. The result of qualitative classification for 15 batches of "YIQING" tablets using principle component analysis was consistent with the quantitative fingerprint assessment using the average linear quantitative fingerprint method. Therefore, chemometrics, multiwavelength total fusion profiling in conjunction with average linear quantitative fingerprint method and antioxidant activity can control the quality of traditional Chinese medicines/herbal preparations comprehensively and practically. |
Databáze: | OpenAIRE |
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