In vivo, noncontact, real-time, PV[O]H imaging of the immediate local physiological response to spinal cord injury in a rat model
| Autor: | Charles M. Peterson, Steve Ortiz, Jerry Goodisman, Seth Fillioe, Ricky McDonough, John J. I. Kim, Kyle Kelly Bishop, Alexander Vincent Struck Jannini, Julie M. Hasenwinkel, Joseph Chaiken |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Paper
Diagnostic Imaging Cord Materials science Infrared Rays hematocrit Rat model Biomedical Engineering 01 natural sciences law.invention 010309 optics Biomaterials Rats Sprague-Dawley In vivo law Computer Systems 0103 physical sciences Image Interpretation Computer-Assisted medicine Animals Spinal cord injury Spinal Cord Injuries Blood Volume Special Section on Biomedical Imaging and Sensing imaging medicine.disease Laser Spinal cord Photobleaching Atomic and Molecular Physics and Optics spinal cord injury turbidity Electronic Optical and Magnetic Materials PV[O]H Rats in vivo medicine.anatomical_structure Models Animal Female Preclinical imaging Algorithms Biomedical engineering |
| Zdroj: | Journal of Biomedical Optics |
| ISSN: | 1560-2281 1083-3668 |
| Popis: | We report a small exploratory study of a methodology for real-time imaging of chemical and physical changes in spinal cords in the immediate aftermath of a localized contusive injury. One hundred separate experiments involving scanning NIR images, one-dimensional, two-dimensional (2-D), and point measurements, obtained in vivo, within a 3×7 mm field, on spinal cords surgically exposed between T9 and T10 revealed differences between injured and healthy cords. The collected raw data, i.e., elastic and inelastic emission from the laser probed tissues, combined via the PV[O]H algorithm, allow construction of five images over the first 5 h post injury. Within the larger study, a total of 13 rats were studied using 2-D images, i.e., injured and control. A single 830-nm laser (100-μm diameter round spot) was spatially line-scanned across the cord to reveal photobleaching effects and surface profiles possibly locating a near surface longitudinal artery/vein. In separate experiments, the laser was scanned in two dimensions across the exposed cord surface relative to the injury in a specific pattern to avoid uneven photobleaching of the imaged tissue. The 2-D scanning produced elastic and inelastic emission that allowed construction of PV[O]H images that had good fidelity with the visually observed surfaces and separate line scans and suggested differences between the volume fractions of fluid and turbidity of injured and healthy cord tissue. |
| Databáze: | OpenAIRE |
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