Characterization of a novel 8R,11S-linoleate diol synthase from Penicillium chrysogenum by identification of its enzymatic products
| Autor: | Deok-Kun Oh, Kyung-Chul Shin, Min-Ju Seo |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
hydroxy fatty acid Linoleic acid 030106 microbiology Diol QD415-436 Penicillium chrysogenum Biochemistry Catalysis 03 medical and health sciences chemistry.chemical_compound Endocrinology Reproduction Asexual Research Articles chemistry.chemical_classification Life Cycle Stages 8R 11S-dihydroxy-9 12(Z Z)-octadecadienoic acid biology ATP synthase Linoleate diol synthase Fatty acid Stereoisomerism Cell Biology biology.organism_classification 030104 developmental biology Enzyme Linoleic Acids precocious sexual inducer factor chemistry Penicillium Oxygenases biology.protein Chromatography Liquid |
| Zdroj: | Journal of Lipid Research, Vol 57, Iss 2, Pp 207-218 (2016) Journal of Lipid Research |
| ISSN: | 0022-2275 |
| DOI: | 10.1194/jlr.m061341 |
| Popis: | To identify novel fatty acid diol synthases, putative candidate sequences from Penicillium species were analyzed, and hydroxy fatty acid production by crude Penicillium enzyme extracts was assessed. Penicillium chrysogenum was found to produce an unknown dihydroxy fatty acid, a candidate gene implicated in this production was cloned and expressed, and the expressed enzyme was purified. The product obtained by the reaction of the purified enzyme with linoleic acid was identified as 8R,11S-dihydroxy-9,12(Z,Z)-octadecadienoic acid (8R,11S-DiHODE). The catalytic efficiency of this enzyme toward linoleic acid was the highest among the unsaturated fatty acids tested, indicating that this enzyme was a novel 8R,11S-linoleate diol synthase (8R,11S-LDS). A sexual stage in the life cycle of P. chrysogenum has recently been discovered, and 8R,11S-DiHODE produced by 8R,11S-LDS may constitute a precocious sexual inducer factor, responsible for regulating the sexual and asexual cycles of this fungus. |
| Databáze: | OpenAIRE |
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