Circular RNA CircHIPK3 Promotes Gemcitabine Sensitivity in Bladder Cancer
| Autor: | Ning Zhao, Hui Zhang, Fang Xie, Dalong Xie |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
circHIPK3 medicine.medical_treatment chemotherapy Flow cytometry 03 medical and health sciences 0302 clinical medicine Medicine Cytotoxicity Chemotherapy Bladder cancer medicine.diagnostic_test business.industry Cell growth gemcitabine circular RNA medicine.disease Gemcitabine 030104 developmental biology Real-time polymerase chain reaction Oncology Apoptosis 030220 oncology & carcinogenesis Cancer research bladder cancer business Research Paper medicine.drug |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| DOI: | 10.7150/jca.39722 |
| Popis: | Purpose: Recent studies showed circular RNA (circRNA) played important regulatory roles in tumors, including genesis of chemotherapy resistance. In this study, the role of circHIPK3 on chemotherapy resistance of bladder cancer (BC) will be clarified. Methods: Real-time quantitative PCR was applied to examine the circHIPK3 expression. The gemcitabine sensitivity and cell proliferation viability were analyzed by Cell Counting Kit-8 assay. Double-stained flow cytometry was used to detect the cell apoptosis. Results: In BC tissues and cell lines, the circHIPK3 expression was down-regulated. Its expression had a negative correlation with pathological grade, lymph node metastasis and gemcitabine insensitivity of BC patients. CircHIPK3 was a independent prognostic biomarker for BC patients. The expression of circHIPK3 in T24/gem and J82/gem cell lines (resistant to gemcitabine) was down-regulated significantly. The over-expression of circHIPK3 decreased IC50 of gemcitabine and promoted gemcitabine's cytotoxicity in T24/gem and J82/gem cells. Conclusions: The circHIPK3 is low-expressed in BC and is an independent prognostic biomarker for BC patients. The low-expression of circHIPK3 is associated with the insensitivity to gemcitabine of BC patients, over-expression of circHIPK3 promotes gemcitabine sensitivity in BC. |
| Databáze: | OpenAIRE |
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