Quantitation of cat immunoglobulins in the hemolymph of cat fleas (Siphonaptera: Pulicidae) after feeding on blood
| Autor: | Gary M. Silver, Rex E. Thomas, Jefferson A. Vaughan, Nancy Wisnewski, Abdu F. Azad |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Flea animal diseases Ribulose-Bisphosphate Carboxylase Immunoglobulin G Microbiology Hemolymph Ingestion Animals Ctenocephalides General Veterinary biology Felis fungi Feeding Behavior bacterial infections and mycoses biology.organism_classification Blood meal Pulicidae Infectious Diseases Insect Science Immunology biology.protein Cats Siphonaptera Parasitology Female |
| Zdroj: | Journal of medical entomology. 35(4) |
| ISSN: | 0022-2585 |
| Popis: | Passage of ingested cat immunoglobulin G (IgG) into the hemocoel of cat fleas, Ctenocephalides felis (Bouché), was examined using antibody capture enzyme-linked immunosorbent assays (ELISA) and Western blotting. Fleas were fed heparinized cat blood via membrane feeders. Cat IgG was present in the hemolymph of engorged female fleas 1 h after ingestion at an estimated quantity of 35 +/- 14 micrograms/ml. The prevalence of fleas with demonstrable cat IgG in their hemolymph 1 h after feeding was 100% for both female and male fleas. Following a single blood meal, cat IgG was present in the hemolymph of all 15 fleas tested 1 h after ingestion but dissipated below detectable levels in 10 of 20 fleas examined 3 h after ingestion, and was detectable in only 1 of 10 fleas examined 18 h after ingestion. However, when fleas were provided with continual access to blood over a 72-h period, IgG content in hemolymph, as measured in excised, triturated legs of individual fleas, remained fairly constant (3-16 pg IgG per sample). Flea feeding studies using specific antisera indicated that IgG in flea hemolymph retained its binding activity, and that at least a portion of the IgG was intact. Passage of ingested host antibody from gut into hemocoel is a prerequisite for the possible development of antiflea vaccines that target antigens outside of the flea midgut lumen (e.g., key components of the flea endocrine system controlling oogenesis). |
| Databáze: | OpenAIRE |
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