Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes
| Autor: | M.A. Van Dilla, J.C. Fuscoe, Lynn M. Clark |
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| Rok vydání: | 1986 |
| Předmět: |
Male
Biology law.invention Cell Line chemistry.chemical_compound law Genetics Chromosomes Human Humans Genomic library Cloning Molecular Molecular Biology Metaphase Genetics (clinical) Skin Cloning B chromosome Infant Newborn Chromosome Nucleic Acid Hybridization DNA Flow Cytometry chemistry Recombinant DNA Human genome |
| Zdroj: | Cytogenetics and cell genetics. 43(1-2) |
| ISSN: | 0301-0171 |
| Popis: | We report the construction of 15 human chromosome-specific DNA libraries. Metaphase chromosomes were purified by flow sorting and the DNA was extracted and cleaved with Hindlll before cloning into the λ vector Charon 21 A. A sensitive miniblot hybridization method was used to monitor the physical and biochemical steps in the cloning procedure. Using this method, we have developed a highly efficient protocol for producing large numbers of recombinant phage from 0.2–1.0 × 106 sorted chromosomes. DNA from the following chromosomes was cloned: #4, 6, 8, 9, 11, 13, 14+15, 16, 17, 18, 19, 20, 21, 22 and Y. These libraries are available to the scientific research community and will be valuable in the genetic analysis of the human genome. |
| Databáze: | OpenAIRE |
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