Contribution of iron and protein contents from rat brain subcellular fractions to MR phase imaging
| Autor: | Kai Zhong, Liane Hilfert, K.-H. Smalla, Oliver Speck, Tobias Leutritz, U. Busse |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
chemistry [Iron]
Magnetic Resonance Spectroscopy Iron metabolism [White Matter] methods [Image Interpretation Computer-Assisted] 030218 nuclear medicine & medical imaging White matter 03 medical and health sciences Myelin methods [Magnetic Resonance Imaging] 0302 clinical medicine Nuclear magnetic resonance pathology [Brain] In vivo Image Interpretation Computer-Assisted chemistry [Proteins] medicine Animals Radiology Nuclear Medicine and imaging Microscopy Phase-Contrast ddc:610 Rats Wistar diagnostic imaging [Brain] Chromatography medicine.diagnostic_test Chemistry Brain Proteins Magnetic resonance imaging Nuclear magnetic resonance spectroscopy Magnetic susceptibility Magnetic Resonance Imaging White Matter Rats medicine.anatomical_structure Membrane Anisotropy 030217 neurology & neurosurgery Algorithms Macromolecule Subcellular Fractions |
| Zdroj: | Magnetic Resonance in Medicine Magnetic resonance in medicine 77(5), 2028-2039 (2016). doi:10.1002/mrm.26288 |
| DOI: | 10.1002/mrm.26288 |
| Popis: | Purpose Investigation of magnetic susceptibility and chemical exchange as sources of MRI phase contrast between gray and white matter resulting from protein and iron content from subcellular fractions. Methods This study analyzes the iron and macromolecule content of different subcellular fractions from rat brain and their relation to the water-resonance frequency by NMR spectroscopy. Additionally, the contributions of susceptibility and exchange were determined with different NMR reference substances. Results Only weak correlations between iron (r = 0.4318, P = 0.76) or protein content (r = 0.4704, P = 0.70) and frequency shift were observed. After membrane depletion, the correlation for iron increased to r = -0.9006 (P = 0.0009), whereas the shift relative to protein content increased much less (r = -0.4982, P = 0.64). Exchange-driven frequency shifts were 1.283 ppb/(mg/ml) for myelin and 0.775 ppb/(mg/ml) for synaptosomes; susceptibility-driven shifts were -1.209 ppb/(mg/ml) for myelin and -0.368 ppb/(mg/ml) for synaptosomes. The ratios between susceptibility and exchange differ significantly from simple protein solutions. Conclusions As a result of counteracting susceptibility and exchange and increased relative shifts in membrane-depleted fractions, we conclude that tissue microstructure accounts more for the in vivo phase contrast than in the situation of homogenized tissue. Thus, membranes may generate much of the in vivo MR phase contrast resulting from anisotropy. Magn Reson Med 77:2028-2039, 2017. © 2016 International Society for Magnetic Resonance in Medicine. |
| Databáze: | OpenAIRE |
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