Sugar Uptake and Transport in Rice Embryo. Expression of Companion Cell-Specific Sucrose Transporter (OsSUT1) Induced by Sugar and Light
| Autor: | Pierdomenico Perata, Toshikazu Saitoh, Toshiro Hirose, Ryu Ohsugi, Chiaki Matsukura, Junji Yamaguchi |
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| Rok vydání: | 2000 |
| Předmět: |
Sucrose
Light Physiology Starch Germination Plant Science Biology Carbohydrate metabolism chemistry.chemical_compound Gene Expression Regulation Plant Genetics Sugar In Situ Hybridization Plant Proteins Oryza sativa Membrane Transport Proteins food and beverages Biological Transport Oryza Carbohydrate Blotting Northern Vascular bundle chemistry Biochemistry Seeds Carbohydrate Metabolism Phloem Carrier Proteins Plant Structures Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.124.1.85 |
| Popis: | We investigated sugar uptake and transport in rice (Oryza sativa) embryo during grain germination. Endogenous sugar levels, accumulation of starch granules, and gene expression of a rice sucrose transporter (OsSUT1) were examined using rice embryos germinated with or without exogenous sugar supply. Starch granules remarkably accumulated in the cells around vascular bundles as a consequence of the sugar taken up by the embryos, indicating that the taken-up sugars are transiently converted into starch. In situ detection for OsSUT1 mRNA indicated its localization in the phloem companion cells. Furthermore, northern-blot and in situ hybridization analyses showed that OsSUT1 expression is not detectable in embryos subjected to sugar starvation conditions, whereas its expression is enhanced by an increased endogenous sugar level. Overall results indicate that the expression of companion cell-specific sucrose transporter, OsSUT1 is regulated by the endogenous sugar status as well as light exposure. |
| Databáze: | OpenAIRE |
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