MicroRNA-26a induces osteosarcoma cell growth and metastasis via the Wnt/β-catenin pathway
| Autor: | Wei Qi, Feng Qu, Yu‑Jie Liu, Chunbao Li, Bang‑Tuo Yuan, Jiang-Tao Wang, Hong‑Liang Li, Gang Zhao, Xue‑Zhen Shen |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Regulation of gene expression Genetics Cancer Research Oncogene Wnt signaling pathway Articles Transfection Biology Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology 030220 oncology & carcinogenesis Catenin microRNA Ectopic expression Viability assay |
| Zdroj: | Oncology Letters. 11:1592-1596 |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2015.4073 |
| Popis: | MicroRNAs (miRNAs/miRs) are a type of highly conserved, small non-coding RNA that are vital to the post-transcriptional regulation of gene expression via base pairing with target mRNA 3′-untranslated regions (3′-UTRs). Several studies have indicated that the abnormal expression of miRNAs occurs frequently in human osteosarcoma (OS). In the present study, the role of miR-26a in the progression and metastasis of OS was investigated using reverse transcription-quantitative polymerase chain reaction, a luciferase activity assay, cell viability assay, in vitro migration and invasion assays, transfection and western blot analysis. miR-26a was upregulated in OS tissues and cell lines, and the expression of miR-26a was indicated to affect the proliferation, migration and invasion of OS Saos-2 cells. At the molecular level, the results showed that glycogen synthase kinase-3β (GSK-3β) was identified as a target of miR-26a, and the ectopic expression of miR-26a inhibited GSK-3β by directly binding to the 3′-UTR. Therefore, the expression of miR-26a was negatively correlated with GSK-3β in the OS tissues. These data suggest that miR-26a is significant in the proliferation of human OS cells due to the direct regulation of Wnt/β-catenin signaling. |
| Databáze: | OpenAIRE |
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