Structure Characterization of Phospholipids and Lipid a of Pseudomonas Putida KT2442
| Autor: | Jianli Wang, Wang Yuqian, Li Ye, Xiaoyuan Wang, Guanjun Tao, Biwen Wang |
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| Rok vydání: | 2015 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Cardiolipins Electrospray ionization Hydroxylation Ammonium Chloride Polyhydroxyalkanoates Lipid A chemistry.chemical_compound Cardiolipin Chromatography High Pressure Liquid Phospholipids Spectroscopy Phosphatidylethanolamine Chromatography Molecular Structure biology Pseudomonas putida Hydrolysis Phosphatidylethanolamines General Medicine biology.organism_classification Atomic and Molecular Physics and Optics Thin-layer chromatography chemistry Biochemistry lipids (amino acids peptides and proteins) Chromatography Thin Layer |
| Zdroj: | European Journal of Mass Spectrometry. 21:739-746 |
| ISSN: | 1751-6838 1469-0667 |
| Popis: | Pseudomonas putida KT2442 is an important bacterium for producing various types of polyhydroxyalkanoate polymers. Phospholipids and lipid A in membranes of P. putida play important roles in stress responses, but detailed structural information of these lipids is not known. In this study, phospholipids and lipid A were isolated from P. putida KT2442, and their structures were analyzed using thin layer chromatography, high performance liquid chromatography and electrospray ionization/mass spectrometry. Major phospholipids in P. putida KT2442 were phosphatidylethanolamine (79.9%), phosphatidylglycero1 (12.7%) and cardiolipin (7.4%), with C16:1 and/or C18:1 acyl chains. Four lipid A species were found in P. putida KT2442: two are hexa-acylated and the other two are penta-acylated. Compared with lipid A of P. aeruginosa, P. putida lipid A has less hydroxylation on the secondary acyl chains and less modification. Therefore, P. putida lipid A could be used as a base structure to investigate lipid A modification of P. aeruginosa for understanding its pathogenesis. |
| Databáze: | OpenAIRE |
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