Antioxidant role of PRGF on RPE cells after blue light insult as a therapy for neurodegenerative diseases

Autor: Jesus Merayo-Lloves, Eduardo Anitua, Luis Fernández-Vega, Carlota Suarez-Barrio, Maria de la Fuente, Begoña Baamonde-Arbaiza, Eva García-Pérez, Susana del Olmo-Aguado, Luis Fernández-Vega-Cueto, Francisco Muruzabal
Rok vydání: 2020
Předmět:
0301 basic medicine
antioxidant
Antioxidant
Light
medicine.medical_treatment
Retinal Pigment Epithelium
Pharmacology
medicine.disease_cause
Antioxidants
lcsh:Chemistry
Plasma
chemistry.chemical_compound
neurodegenerative disease
0302 clinical medicine
retinal pigment epithelium (RPE)
lcsh:QH301-705.5
Spectroscopy
Neurodegenerative Diseases
General Medicine
Mitochondria
Computer Science Applications
Age-related macular degeneration (AMD)
Intercellular Signaling Peptides and Proteins
Female
ARPE19
Phototoxicity
Oxidation-Reduction
Signal Transduction
Adult
Cell Survival
reactive oxygen species (ROS)
Article
Retina
Catalysis
Cell Line
Inorganic Chemistry
03 medical and health sciences
medicine
Animals
Humans
Rats
Wistar
Physical and Theoretical Chemistry
Molecular Biology
business.industry
Organic Chemistry
plasma rich in growth factors (PRGF)
Epithelial Cells
Retinal
Glutathione
Macular degeneration
medicine.disease
blue light
KEAP1
Rats
Oxidative Stress
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
030221 ophthalmology & optometry
Reactive Oxygen Species
business
Ex vivo
Oxidative stress
Zdroj: Scopus
RUO. Repositorio Institucional de la Universidad de Oviedo
instname
International Journal of Molecular Sciences
Volume 21
Issue 3
RUO: Repositorio Institucional de la Universidad de Oviedo
Universidad de Oviedo (UNIOVI)
International Journal of Molecular Sciences, Vol 21, Iss 3, p 1021 (2020)
Popis: Oxidative stress has a strong impact on the development of retinal diseases such as age-related macular degeneration (AMD). Plasma rich in growth factors (PRGF) is a novel therapeutic approach in ophthalmological pathologies. The aim of this study was to analyze the antioxidant effect of PRGF in retinal epithelial cells (EPR) in in vitro and ex vivo retinal phototoxicity models. In vitro analyses were performed on ARPE19 human cell line. Viability and mitochondrial status were assessed in order to test the primary effects of PRGF. GSH level, and protein and gene expression of the main antioxidant pathway (Keap1, Nrf2, GCL, HO-1, and NQO1) were also studied. Ex vivo analyses were performed on rat RPE, and HO-1 and Nrf2 gene and protein expression were evaluated. The results show that PRGF reduces light insult by stimulating the cell response against oxidative damage and modulates the antioxidant pathway. We conclude that PRGF&rsquo
s protective effect could prove useful as a new therapy for treating neurodegenerative disorders such as AMD.
Databáze: OpenAIRE
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