Staining with chromoxane cyanine R
| Autor: | George Clark |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Cell Nucleus
Aqueous solution Chromatography Ethanol Time Factors Eosin Staining and Labeling Hydrogen bond Benzenesulfonates H&E stain Acetylation Hydrogen-Ion Concentration Stain Ferric Compounds Methylation Staining Rats chemistry.chemical_compound chemistry Spinal Cord Animals Anatomy Hydrate Myelin Sheath |
| Zdroj: | Stain technology. 54(6) |
| ISSN: | 0038-9153 |
| Popis: | Since Pearse in 1957 introduced chromoxane cyanine R as a dual nuclear and cytoplasmic stain there have appeared numerous procedures for use of this dye. These have differed widely, sharing in common mainly the implication that each is best. A defendable procedure has been developed on an experimental basis and is reported here. Four stock solutions are needed: (1) a 0.2% solution of chromoxane cyanine R in 0.5% aqueous H2SO4 (v/v); boil this solution for 5 min, (2) 10% FeCl3 in 3% HCl, (3) 1% aqueous HN4OH, and (4) 1% HCl in 70% ethanol. The staining solution: 40 ml of dye solution, 2 ml of FeCl3 solution, 8 ml H2O. Dewax and hydrate sections and stain for 10 min. If a myelin sheath stain is desired differentiate for 1 min in solution (3). For a nuclear stain differentiate for 1 min in solution (4). The nuclear stain when counterstained with eosin closely resembles the routine hematoxylin and eosin. Histochemical tests show that the functional group for myelin staining contains nitrogen, and probably hydrogen bonding is involved. The nuclear stain involves a different functional group and possibly neither electrostatic nor hydrogen bonding. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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