Cobalt-protoporphyrin enhances heme oxygenase 1 expression and attenuates liver ischemia/reperfusion injury by inhibiting apoptosis
| Autor: | Guoliang Zhang, Jiang Li, Jin-Zhen Cai, Jing Li, Dahong Teng, Junjie Li, Xiao-Ye Sun, Bin Wu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research H&E stain Aspartate transaminase Protoporphyrins Apoptosis Biochemistry 03 medical and health sciences Mice 0302 clinical medicine Genetics medicine Animals Aspartate Aminotransferases Molecular Biology TUNEL assay biology Chemistry Caspase 3 Alanine Transaminase medicine.disease Molecular biology COPP Immunohistochemistry Up-Regulation Heme oxygenase Mice Inbred C57BL Disease Models Animal 030104 developmental biology Oncology Terminal deoxynucleotidyl transferase Liver Proto-Oncogene Proteins c-bcl-2 030220 oncology & carcinogenesis Reperfusion Injury biology.protein Molecular Medicine Reperfusion injury Heme Oxygenase-1 |
| Zdroj: | Molecular medicine reports. 17(3) |
| ISSN: | 1791-3004 |
| Popis: | The aim of the present study was to investigate the preconditioning effect and underlying mechanisms of cobalt-protoporphyrin (CoPP) in a mouse model of liver ischemia‑reperfusion (I/R) injury. Mice were divided into five groups: Sham‑operated (control), I/R, I/R + CoPP, I/R + CoPP and zinc‑protoporphyrin (ZnPP) and I/R + ZnPP. Serum levels of aspartate transaminase (AST) and alanine aminotransferase (ALT) were detected using commercial kits. The expression of the pro‑apoptotic protein caspase‑3 was detected by immunohistochemistry and the expression levels of the anti‑apoptotic protein B‑cell lymphoma 2 (Bcl‑2) and heme oxygenase 1 (HO‑1) were analyzed by western blotting. Sections of liver tissue were stained with hematoxylin and eosin to observe pathologic alterations. Furthermore, hepatocyte apoptosis was detected using a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. AST and ALT levels of the CoPP preconditioned group were significantly reduced compared with the IR injury group (P |
| Databáze: | OpenAIRE |
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