Developmental patterns of caspase-3, bax and bcl-2 proteins expression in the human spinal ganglia
| Autor: | Katarina Vukojević, Dominko Carev, Mirna Saraga-Babić, Danijel Petrovič, Damir Sapunar |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Physiology Cellular differentiation Population Fluorescent Antibody Technique Caspase 3 Gestational Age apoptosis bcl-2 bax caspase-3 spinal ganglia development human embryos Andrology Bcl-2-associated X protein Ganglia Spinal medicine Humans education bcl-2-Associated X Protein education.field_of_study Microscopy biology Cell Biology General Medicine Immunohistochemistry Ganglion Enzyme Activation medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Apoptosis biology.protein Developmental biology |
| Zdroj: | Journal of molecular histology. 39(3) |
| ISSN: | 1567-2379 |
| Popis: | The distribution of the bcl-2, bax and caspase-3 proteins was investigated in the cells of developing human spinal ganglia. Paraffin sections of 10 human conceptuses between 5th and 9th gestational weeks were analysed morphologically, immunohistochemically and by TUNEL-method. Cells positive to caspase-3 had brown stained nuclei or nuclear fragmentations. At earliest stages, 6% of ganglion population were caspase-3 positive cells. Later on, a significant increase in number of caspase-3 positive cells appeared, particularly in the ventral part of ganglia (12%), and subsequently decreased to 6%. TUNEL-positive cells had the same distribution pattern as caspase-3 positive cells. Bax-positive cells followed the developmental pattern similar to caspase-3 cells, changing in range between 20% and 32%. There were 8% of bcl-2 positive cells at earliest stages. They increased significantly in dorsal part of the ganglion during the 7th week (28%), and than dropped to 15% by the end of the 8th week. These findings suggest a ventro-dorsal course of development in human spinal ganglia. Number of bcl-2, bax and caspase-3 positive cells changed in a temporally and spatially restricted manner, coincidently with ganglion differentiation. While apoptosis might control cell number, bcl-2 could act in suppression of apoptosis and enhancement of cell differentiation. |
| Databáze: | OpenAIRE |
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