Biomechanical Testing of Murine Tendons
| Autor: | Stavros Thomopoulos, Mikhail Golman, Iden Kurtaliaj, Adam C. Abraham |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
musculoskeletal diseases
Male General Chemical Engineering Tendon disorders Biomechanical testing Achilles Tendon Article General Biochemistry Genetics and Molecular Biology Mice Imaging Three-Dimensional Medicine Animals Humerus Surgical repair General Immunology and Microbiology business.industry General Neuroscience Biomechanics Reproducibility of Results Enthesis musculoskeletal system Tendon Biomechanical Phenomena Mice Inbred C57BL medicine.anatomical_structure Models Animal Printing Three-Dimensional Female Calcaneus business Biomedical engineering |
| Zdroj: | J Vis Exp |
| ISSN: | 1940-087X |
| Popis: | Tendon disorders are common, affect people of all ages, and are often debilitating. Standard treatments, such as anti-inflammatory drugs, rehabilitation, and surgical repair, often fail. In order to define tendon function and demonstrate efficacy of new treatments, the mechanical properties of tendons from animal models must be accurately determined. Murine animal models are now widely used to study tendon disorders and evaluate novel treatments for tendinopathies; however, determining the mechanical properties of mouse tendons has been challenging. In this study, a new system was developed for tendon mechanical testing that includes 3D-printed fixtures that exactly match the anatomies of the humerus and calcaneus to mechanically test supraspinatus tendons and Achilles tendons, respectively. These fixtures were developed using 3D reconstructions of native bone anatomy, solid modeling, and additive manufacturing. The new approach eliminated artifactual gripping failures (e.g., failure at the growth plate failure rather than in the tendon), decreased overall testing time, and increased reproducibility. Furthermore, this new method is readily adaptable for testing other murine tendons and tendons from other animals. |
| Databáze: | OpenAIRE |
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