Tomato Root Penetration in Soil Requires a Coaction between Ethylene and Auxin Signaling
| Autor: | Parankusam Santisree, Rameshwar Sharma, Maria G. Ivanchenko, Yellamaraju Sreelakshmi, Sapana Nongmaithem, Himabindu Vasuki |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Cyclopropanes
Physiology Meristem Gravitropism Plant Science Biology Genes Plant Mechanotransduction Cellular Plant Roots Soil Solanum lycopersicum Gene Expression Regulation Plant Auxin Botany Genetics chemistry.chemical_classification Indoleacetic Acids Development and Hormone Action fungi food and beverages Positive gravitropism Biological Transport Penetration (firestop) Ethylenes Horticulture chemistry Seedlings Germination Mutation Imbibition Polar auxin transport Signal Transduction |
| Zdroj: | Plant Physiology. 156:1424-1438 |
| ISSN: | 1532-2548 |
| DOI: | 10.1104/pp.111.177014 |
| Popis: | During seed germination, emerging roots display positive gravitropism and penetrate into the soil for nutrition and anchorage. Tomato (Solanum lycopersicum) seeds germinated in the presence of 1-methylcyclopropene (1-MCP), an inhibitor of ethylene action, failed to insert roots into Soilrite and grew in the air, forming loops. Time-lapse video imaging showed that 1-MCP-grown root tips retained positive gravitropism and made contact with the surface of Soilrite but failed to penetrate into the Soilrite. Time-course studies revealed that the effect of 1-MCP was most prominent when seed imbibition and germination were carried out in the continual presence of 1-MCP. Conversely, 1-MCP was ineffective when applied postgermination after penetration of roots in the Soilrite. Furthermore, treatment with 1-MCP caused a reduction in DR5::β-glucuronidase auxin-reporter activity and modified the expression of SlIAA3 and SlIAA9 transcripts, indicating interference with auxin signaling. The reduced ethylene perception mutant, Never-ripe, displayed decreased ability for root penetration, and the enhanced polar auxin transport mutant, polycotyledon, showed a nearly normal root penetration in the presence of 1-MCP, which could be reversed by application of auxin transport inhibitors. Our results indicate that during tomato seed germination, a coaction between ethylene and auxin is required for root penetration into the soil. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|