Identification of MICA as a new polymorphic alloantigen recognized by antibodies in sera of organ transplant recipients
| Autor: | Fariba Mirbaha, Yizhou Zou, Norberto Walter Zwirner, Cintia Y. Marcos, Peter Stastny |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Isoantigens
medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Recombinant Fusion Proteins Molecular Sequence Data Immunology Cell Inmunología Human leukocyte antigen Major histocompatibility complex Organ transplantation law.invention ALIOANTIGEN Isoantibodies law MHC class I medicine Animals Humans Immunology and Allergy Amino Acid Sequence Polymorphism Genetic biology TRANSPLANTATION Histocompatibility Antigens Class I Organ Transplantation General Medicine Kidney Transplantation Molecular biology HLA Transplantation Medicina Básica stomatognathic diseases medicine.anatomical_structure MICA Recombinant DNA biology.protein Rabbits Antibody HeLa Cells |
| Zdroj: | Human Immunology. 61:917-924 |
| ISSN: | 0198-8859 |
| DOI: | 10.1016/s0198-8859(00)00162-2 |
| Popis: | MHC class I-related chain A (MICA) is an HLA-related, polymorphic gene the product of which may be recognized by a subpopularion of intestinal γδ T cells and may play a role in the activation of a subpopulation of natural killer cells. Using anti-MICA specific rabbit sera we previously demonstrated that freshly isolated monocytes, keratinocytes, fibroblasts, and endothelial cells express MICA. To analyze whether MICA may be a target for specific antibodies in sera of transplanted patients, we produced three recombinant MICA proteins consisting of the α1, α2, and α3 domains, and used them in an enzyme-linked immunosorbent assay. We found that several patients had specific antibodies against MICA. Most of them were detected in serum samples collected at different times after organ rejection. Although this finding raises the question of how these patients became immunized, the fact that the polymorphic, HLA-like MICA molecule, expressed at the cell surface of endothelial cells, is recognized by specific antibodies in sera of transplanted patients, suggests the MICA may be a target molecule in allograft rejection. Fil: Zwirner, Norberto Walter. University of Texas; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marcos, Cintia Y.. University of Texas; Estados Unidos Fil: Mirbaha, Fariba. University of Texas; Estados Unidos Fil: Zou, Yizhou. University of Texas; Estados Unidos Fil: Stastny, Peter. University of Texas; Estados Unidos |
| Databáze: | OpenAIRE |
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