Effects of water-soluble tomato concentrate on platelet aggregation
| Autor: | Fulong Liao, Shi-Lan Ding, Jin-Yu Wang, Qiong-Ling Zhang, Yun You, Lan Wang, Xu-Guang Zhang, Ying Chen, Qian Zhang, Lei Liu, Ya-Hong Wang, Rixin Liang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
lcsh:R5-920
medicine.diagnostic_test Chemistry watersoluble tomato concentrate gp iib/iiia pdi Pharmacology Fibrinogen In vitro Adenosine diphosphate chemistry.chemical_compound Complementary and alternative medicine Western blot pecam-1 platelet aggregation medicine Platelet Platelet activation Protein disulfide-isomerase pi3k lcsh:Medicine (General) Ex vivo medicine.drug |
| Zdroj: | World Journal of Traditional Chinese Medicine, Vol 5, Iss 4, Pp 260-268 (2019) |
| ISSN: | 2311-8571 |
| Popis: | Objective: To investigate the antiplatelet aggregation effect of water-soluble tomato concentrate (WSTC) and explore the underlying molecular mechanisms. Materials and Methods: Platelet aggregometry was used to quantify rat platelet aggregation with the maximum aggregation rate in vitro and ex vivo. Then, the fibrinogen (FIB) binding assay was employed to detect the effect of WSTC on the activation of platelet integrin αIIβ3 (GP IIb/IIIa). Furthermore, Western blot was performed to assess the platelet protein levels of phosphoinositide 3-kinase 110 β (PI3K110 β), protein disulfide isomerase (PDI), platelet endothelial cell adhesion molecule 1 (PECAM-1), and β1-Tubulin. Results: WSTC inhibited adenosine diphosphate (ADP) and collagen-induced platelet aggregation in a concentration-dependent manner in vitro, at IC50values of 3.05 g/L and 8.03 g/L, respectively. Significantly reduced ex vivo ADP induced platelet aggregation was observed after oral consumption of WSTC for 4 weeks in rats; average inhibition rates were 24.42%, 21.48%, and 20.87% for 25 mg/Kg, 75 mg/Kg, and 150 mg/Kg WSTC, respectively. It appeared that WSTC had no influence on coagulation function in rats. Incubation with WSTC decreased FIB binding to GP IIb/IIIa by 17.47% and 32.29% at the concentrations of 0.6 and 6 g/L, respectively. WSTC at 0.6 and 6 g/L markedly downregulated PI3K110 β, PDI, and PECAM-1 in platelets, and upregulated β1-Tubulin, in a concentration-dependent manner. Conclusion: WSTC inhibits platelet activation through modulation of platelet skeletal stability and suppresses GP IIb/IIIa receptor-mediated platelet aggregation, likely via the PI3K signaling pathway and PDI inhibition. |
| Databáze: | OpenAIRE |
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