Upregulation of circFLNA contributes to laryngeal squamous cell carcinoma migration by circFLNA–miR-486-3p-FLNA axis
| Autor: | Jianxing Wang, J H Dong, Yan Liu, Hui-Jun Duan, Ou Xu, Xin-Ju Jia, Xiumin Ren, Chun-Guang Shan, Shu-Xia Liu, Hai-Zhong Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
MMP2 Laryngeal squamous cell carcinoma Biology Filamin lcsh:RC254-282 Metastasis 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation microRNA Genetics medicine FLNA lcsh:QH573-671 Migration miRNA lcsh:Cytology Cell migration lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease Oncology Tumor progression 030220 oncology & carcinogenesis Cancer research circRNAs Primary Research |
| Zdroj: | Cancer Cell International Cancer Cell International, Vol 19, Iss 1, Pp 1-13 (2019) |
| ISSN: | 1475-2867 |
| Popis: | Background Accumulating evidence shows that circular RNAs (circRNAs) plays vital roles in tumor progression. However, the biological functions of circRNAs in laryngeal squamous cell carcinoma (LSCC) metastasis is still unclear. Methods qRT-PCR was used to detect circFLNA, miRNAs and FLNA mRNA expression. Transwell assay and western blot were performed to evaluate cell migration ability and to detect FLNA, MMP2 and MLK1 protein expression, respectively. RNA pull-down analysis was used to find the binding-miRNAs of circFLNA. Luciferase reporter assay was used to examine the effect of circFLNA on miRNAs and miR-486-3p on FLNA expression. Results In this study, we confirmed that a Filamin A (FLNA)-derived hsa_circ_0092012 known as circFLNA, was upregulated in LSCC, and the higher expression of circFLNA was correlated with LSCC lymph node metastasis. Increased circFLNA facilitates LSCC cell migration ability through upregulating FLNA and MMP2 protein expression. Mechanistically, we find that circFLNA sponges miR-486-3p in LSCC cells, relieving miR-486-3p-induced repression of FLNA which promotes LSCC cell migration. Accordingly, FLNA mRNA is overexpressed in LSCC tissues and a higher FLNA level is correlated with poor survival. Dysregulation of the circFLNA/miR-486-3p/FLNA regulatory pathway contributes to LSCC migration. Conclusions In summary, our study sheds light on the regulatory mechanism of circFLNA in LSCC migration via sponging miR‐486-3p, which downregulates the FLNA protein expression. Targeting circFLNA/miR-486-3p/FLAN axis provides a potential therapeutic target for aggressive LSCC. |
| Databáze: | OpenAIRE |
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