Eukaryotic initiation factor 2α kinase is a nitric oxide-responsive mercury sensor enzyme: Potent inhibition of catalysis by the mercury cation and reversal by nitric oxide
| Autor: | Marketa Martinkova, Jotaro Igarashi, Toru Shimizu |
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| Rok vydání: | 2007 |
| Předmět: |
Translation
Heme binding Cations Divalent Metal cations Biophysics eIF2α kinase Nitric Oxide Biochemistry Catalysis Nitric oxide Mice eIF-2 Kinase chemistry.chemical_compound Structural Biology Metals Heavy Eukaryotic initiation factor Genetics Protein biosynthesis Animals Kinase activity Molecular Biology chemistry.chemical_classification Dose-Response Relationship Drug Kinase Mercury Cell Biology Protein kinase R Enzyme chemistry Protein Biosynthesis Protein synthesis |
| Zdroj: | FEBS Letters. 581:4109-4114 |
| ISSN: | 0014-5793 |
| Popis: | The activity of one of the eukaryotic initiation factor 2alpha kinases, heme-regulated inhibitor (HRI), is modulated by heme binding. Here, we demonstrate for the first time that Hg2+ strongly inhibits the function of HRI (IC50=0.6 microM), and nitric oxide fully reverses this inhibition. Other divalent metal cations, such as Fe2+, Cu2+, Cd2+, Zn2+ and Pb2+, also significantly inhibit kinase activity with IC50 values of 1.9-8.5 microM. Notably, inhibition by cations other than Hg2+ is not reversed by nitric oxide. Our present data support dual roles of Hg2+ and nitric oxide in the regulation of protein synthesis during cell emergency states. |
| Databáze: | OpenAIRE |
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