Quantitative Evaluation of SARS-CoV-2 Inactivation Using a Deep Ultraviolet Light-Emitting Diode
| Autor: | Takeshi Yasui, Kentaro Nagamatsu, Takahiko Mizuno, Hideki Arimochi, Takaaki Koma, Koji Yasutomo, Akihiro Suzuki, Masako Nomaguchi, Koichiro Tsuchiya, Takeo Minamikawa, Kaoru Matsuoka |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Science viruses Quantitative Evaluations Article 03 medical and health sciences 0302 clinical medicine Basic knowledge Ultraviolet light 030212 general & internal medicine Irradiation skin and connective tissue diseases Diode Infectivity Multidisciplinary Chemistry SARS-CoV-2 fungi virus diseases body regions 030104 developmental biology Viral infection Biophysics Inorganic LEDs Medicine Biophotonics |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) Scientific Reports |
| Popis: | Inactivation technology for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is certainly a critical measure to mitigate the spread of coronavirus disease 2019 (COVID-19). A deep ultraviolet light-emitting diode (DUV-LED) would be a promising candidate to inactivate SARS-CoV-2, based on the well-known antiviral effects of DUV on microorganisms and viruses. However, due to variations in the inactivation effects across different viruses, quantitative evaluations of the inactivation profile of SARS-CoV-2 by DUV-LED irradiation need to be performed. In the present study, we quantify the irradiation dose of DUV-LED necessary to inactivate SARS-CoV-2. For this purpose, we determined the culture media suitable for the irradiation of SARS-CoV-2 and optimized the irradiation apparatus using commercially available DUV-LEDs that operate at a center wavelength of 265, 280, or 300 nm. Under these conditions, we successfully analyzed the relationship between SARS-CoV-2 infectivity and the irradiation dose of the DUV-LEDs at each wavelength without irrelevant biological effects. In conclusion, total doses of 1.8 mJ/cm2 for 265 nm, 3.0 mJ/cm2 for 280 nm, and 23 mJ/cm2 for 300 nm are required to inactivate 99.9% of SARS-CoV-2. Our results provide quantitative antiviral effects of DUV irradiation on SARS-CoV-2, serving as basic knowledge of inactivation technologies against SARS-CoV-2. |
| Databáze: | OpenAIRE |
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