Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial development
Autor: | Mi-Na Kweon, Seung Il Kim, Mi-Na Kim, Yeji Kim, Su-Hyun Lee, Yong-Soo Lee, Yun-Chan Shin, Tae-Young Kim, O. Eunju, Kwang Soon Kim |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
lgr5 RC799-869 Gut flora Feces Mice g protein-coupled receptors Intestine Small Homeostasis Intestinal Mucosa Wnt Signaling Pathway organoids Stem Cells Gastroenterology LGR5 Cell Differentiation Diseases of the digestive system. Gastroenterology Intestinal epithelium Cell biology Infectious Diseases medicine.anatomical_structure Female Stem cell Akkermansia muciniphila Research Article Research Paper inorganic chemicals Microbiology (medical) 030106 microbiology Biology Microbiology digestive system mucin-degrading bacteria 03 medical and health sciences Organoid medicine epithelial development Animals Humans Cell Proliferation intestinal stem cells gut microbiota Mucin Mucins Epithelial Cells Akkermansia biology.organism_classification Fatty Acids Volatile akkermansia muciniphila Small intestine Gastrointestinal Microbiome Mice Inbred C57BL 030104 developmental biology Methotrexate small intestine |
Zdroj: | Gut Microbes, Vol 13, Iss 1 (2021) Gut Microbes article-version (VoR) Version of Record |
ISSN: | 1949-0984 1949-0976 |
Popis: | Mucin-degrading bacteria are densely populated in the intestinal epithelium; however, their interaction with intestinal stem cells (ISCs) and their progeny have not been elucidated. To determine whether mucin-degrading bacteria play a role in gut homeostasis, mice were treated with Akkermansia muciniphila, a specialized species that degrades mucin. Administration of A. muciniphila for 4 weeks accelerated the proliferation of Lgr5+ ISCs and promoted the differentiation of Paneth cells and goblet cells in the small intestine (SI). We found similar effects of A. muciniphila in the colon. The levels of acetic and propionic acids were higher in the cecal contents of A. muciniphila-treated mice than in PBS-treated mice. SI organoids treated with cecal contents obtained from A. muciniphila-treated mice were larger and could be diminished by treatment with G protein-coupled receptor (Gpr) 41/43 antagonists. Pre-treatment of mice with A. muciniphila reduced gut damage caused by radiation and methotrexate. Further, a novel isotype of the A. muciniphila strain was isolated from heathy human feces that showed enhanced function in intestinal epithelial regeneration. These findings suggest that mucin-degrading bacteria (e.g., A. muciniphila) may play a crucial role in promoting ISC-mediated epithelial development and contribute to intestinal homeostasis maintenance. |
Databáze: | OpenAIRE |
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