Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial development

Autor: Mi-Na Kweon, Seung Il Kim, Mi-Na Kim, Yeji Kim, Su-Hyun Lee, Yong-Soo Lee, Yun-Chan Shin, Tae-Young Kim, O. Eunju, Kwang Soon Kim
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
lgr5
RC799-869
Gut flora
Feces
Mice
g protein-coupled receptors
Intestine
Small

Homeostasis
Intestinal Mucosa
Wnt Signaling Pathway
organoids
Stem Cells
Gastroenterology
LGR5
Cell Differentiation
Diseases of the digestive system. Gastroenterology
Intestinal epithelium
Cell biology
Infectious Diseases
medicine.anatomical_structure
Female
Stem cell
Akkermansia muciniphila
Research Article
Research Paper
inorganic chemicals
Microbiology (medical)
030106 microbiology
Biology
Microbiology
digestive system
mucin-degrading bacteria
03 medical and health sciences
Organoid
medicine
epithelial development
Animals
Humans
Cell Proliferation
intestinal stem cells
gut microbiota
Mucin
Mucins
Epithelial Cells
Akkermansia
biology.organism_classification
Fatty Acids
Volatile

akkermansia muciniphila
Small intestine
Gastrointestinal Microbiome
Mice
Inbred C57BL

030104 developmental biology
Methotrexate
small intestine
Zdroj: Gut Microbes, Vol 13, Iss 1 (2021)
Gut Microbes
article-version (VoR) Version of Record
ISSN: 1949-0984
1949-0976
Popis: Mucin-degrading bacteria are densely populated in the intestinal epithelium; however, their interaction with intestinal stem cells (ISCs) and their progeny have not been elucidated. To determine whether mucin-degrading bacteria play a role in gut homeostasis, mice were treated with Akkermansia muciniphila, a specialized species that degrades mucin. Administration of A. muciniphila for 4 weeks accelerated the proliferation of Lgr5+ ISCs and promoted the differentiation of Paneth cells and goblet cells in the small intestine (SI). We found similar effects of A. muciniphila in the colon. The levels of acetic and propionic acids were higher in the cecal contents of A. muciniphila-treated mice than in PBS-treated mice. SI organoids treated with cecal contents obtained from A. muciniphila-treated mice were larger and could be diminished by treatment with G protein-coupled receptor (Gpr) 41/43 antagonists. Pre-treatment of mice with A. muciniphila reduced gut damage caused by radiation and methotrexate. Further, a novel isotype of the A. muciniphila strain was isolated from heathy human feces that showed enhanced function in intestinal epithelial regeneration. These findings suggest that mucin-degrading bacteria (e.g., A. muciniphila) may play a crucial role in promoting ISC-mediated epithelial development and contribute to intestinal homeostasis maintenance.
Databáze: OpenAIRE