Down-regulated miRs specifically correlate with non-cardial gastric cancers and Lauren's classification system

Autor: Danilo Ranieri, V. Canu, Maria Rosaria Torrisi, Stefania Uccini, Giovanni Blandino, Paolo Mercantini, Laura Lorenzon, Claudia Cippitelli, Marco Cavallini, Deborah French, Riccardo Avantifiori
Rok vydání: 2016
Předmět:
0301 basic medicine
Oncology
Male
Pathology
Logistic regression
0302 clinical medicine
mir31
mir375
80 and over
gastric cancer
lauren classification
mir148a
mir204
non-cardial gastric cancer
adenocarcinoma
adult
age factors
aged
aged
80 and over
cell line
tumor
down-regulation
female
gene expression regulation
neoplastic
humans
janus kinase 2
male
micrornas
middle aged
prognosis
protein-serine-threonine kinases
proto-oncogene proteins c-bcl-2
pyruvate dehydrogenase (acetyl-transferring) kinase
roc curve
repressor proteins
retrospective studies
sensitivity and specificity
sex factors
stomach neoplasms
Aged
80 and over
Age Factors
General Medicine
cell line
gene expression regulation
Middle Aged
Prognosis
Gene Expression Regulation
Neoplastic
Proto-Oncogene Proteins c-bcl-2
030220 oncology & carcinogenesis
Adenocarcinoma
Female
Adult
medicine.medical_specialty
tumor
Down-Regulation
Protein Serine-Threonine Kinases
Sensitivity and Specificity
03 medical and health sciences
Sex Factors
Stomach Neoplasms
Internal medicine
Cell Line
Tumor
medicine
Humans
Pathological
Aged
Retrospective Studies
Receiver operating characteristic
business.industry
Advanced stage
Cancer
Pyruvate Dehydrogenase Acetyl-Transferring Kinase
Retrospective cohort study
Janus Kinase 2
medicine.disease
neoplastic
Repressor Proteins
Young age
MicroRNAs
030104 developmental biology
ROC Curve
Surgery
business
Zdroj: Journal of surgical oncology. 116(2)
ISSN: 1096-9098
Popis: Background and Objectives Gastric cancers are usually characterized using Lauren's classification into intestinal and diffuse types. We previously documented the down-modulation of miR31, miR148a, miR204, and miR375 in gastric cancers. We aimed this manuscript to investigate these miRs with the end-points of diagnosis, Lauren's classification and prognosis. Methods A total of 117 resected non-cardial adenocarcinomas were evaluated for miRs' expressions. The performance of miRs’ expressions for cancer diagnosis was tested using ROC curves. Logistic regression was conducted with the end-point of Lauren's classification. Kaplan-Meier and Cox analyses were performed for OS, DFS, and DSS. miRs’ targets were reviewed using PRISMA method and BCL-2 was further investigated in cell lines. Results ROC curves documented that miRs’ down-modulation was significant in differentiating cancer versus normal tissues. Diffuse type cancers were associated with female sex, young age, and miR375 higher expression. We confirmed BCL-2 as a miR204 target. However, survival analyses confirmed the pathologic criteria (advanced stages, LNR, and low LNH) as the significant variables correlated to worse prognosis. Conclusions The down-modulation of miR31, miR148a, miR204, and miR375 is significantly associated with non-cardial gastric cancers and miR375 is specifically linked to Lauren's classification. Nevertheless, standard pathological features display as the independent variables associated with worse prognosis.
Databáze: OpenAIRE
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