The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana
| Autor: | David Honys, Jan Fíla, Zbyněk Zdráhal, Božena Klodová, Miloslav Juříček, David Potěšil, Petr Šesták |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Proteomics Arabidopsis thaliana Mutant Arabidopsis 01 natural sciences Ribosome Transcriptome lcsh:Chemistry Gene Expression Regulation Plant chaperone lcsh:QH301-705.5 Spectroscopy 2. Zero hunger Homozygote food and beverages General Medicine Plants Genetically Modified Computer Science Applications Cell biology Phenotype flower bud transcriptome Seeds Silique Arabidopsis thaliana chaperone flower bud proteome flower bud transcriptome male gametophyte nascent polypeptide-associated complex Protein subunit Plant Development Germination Flowers Biology arabidopsis thaliana Catalysis Article Inorganic Chemistry 03 medical and health sciences Physical and Theoretical Chemistry Molecular Biology Gene Alleles Arabidopsis Proteins Organic Chemistry Wild type RNA male gametophyte flower bud proteome 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 nascent polypeptide-associated complex Mutation 010606 plant biology & botany Molecular Chaperones |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 21, Iss 6, p 2065 (2020) Volume 21 Issue 6 |
| ISSN: | 1422-0067 |
| Popis: | The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, α and β, and was shown to bind to the nascent polypeptides newly emerging from the ribosomes. NAC function was widely described in yeast and several information are also available about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes encoding NACα subunit, and two genes encoding NACβ. Double homozygous mutant in both genes coding for NACβ was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Both NACβ genes were characterized in more detail—the phenotype of the double homozygous mutant was complemented by a functional NACβ copy. Then, both NACβ genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nacβ mutation, the flower buds’ transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NACβ subunits in stress responses, male gametophyte development, and photosynthesis. |
| Databáze: | OpenAIRE |
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