Characterisation of a New Human Alveolar Macrophage-Like Cell Line (Daisy)

Autor: Holly N. Wilkinson, James D. Williamson, Alyn H. Morice, Simon D. Fraser, Yvette A. Hayman, Jesse Vance, Laura R. Sadofsky, Jorge Cervantes, Simon P. Hart
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Pulmonary and Respiratory Medicine
Macrophage
Sialic Acid Binding Ig-like Lectin 1
THP-1 Cells
CD14
Lipopolysaccharide Receptors
CD11c
Antigens
Differentiation
Myelomonocytic
Receptors
Cell Surface
Antigen-Antibody Complex
Alveolar
Flow cytometry
Cell Line
Immunophenotyping
03 medical and health sciences
0302 clinical medicine
Microscopy
Electron
Transmission
Phagocytosis
Antigens
CD
Macrophages
Alveolar
medicine
Humans
THP1 cell line
Lectins
C-Type
RNA
Messenger
030304 developmental biology
0303 health sciences
Microscopy
CD11b Antigen
medicine.diagnostic_test
Chemistry
CD11 Antigens
Flow Cytometry
Cell biology
Mannose-Binding Lectins
030228 respiratory system
Daisy
Cell culture
Tissue Array Analysis
Alveolar macrophage
B7-1 Antigen
Airway Biology
THP-1
Mitogens
Integrin alpha Chains
CD80
Mannose Receptor
Zdroj: Lung
ISSN: 1432-1750
0341-2040
Popis: Purpose There is currently no true macrophage cell line and in vitro experiments requiring these cells currently require mitogenic stimulation of a macrophage precursor cell line (THP-1) or ex vivo maturation of circulating primary monocytes. In this study, we characterise a human macrophage cell line, derived from THP-1 cells, and compare its phenotype to the THP-1 cells. Methods THP-1 cells with and without mitogenic stimulation were compared to the newly derived macrophage-like cell line (Daisy) using microscopy, flow cytometry, phagocytosis assays, antigen binding assays and gene microarrays. Results We show that the cell line grows predominantly in an adherent monolayer. A panel of antibodies were chosen to investigate the cell surface phenotype of these cells using flow cytometry. Daisy cells expressed more CD11c, CD80, CD163, CD169 and CD206, but less CD14 and CD11b compared with mitogen-stimulated THP-1 cells. Unlike stimulated THP-1 cells which were barely able to bind immune complexes, Daisy cells showed large amounts of immune complex binding. Finally, although not statistically significant, the phagocytic ability of Daisy cells was greater than mitogen-stimulated THP-1 cells, suggesting that the cell line is more similar to mature macrophages. Conclusions The observed phenotype suggests that Daisy cells are a good model of human macrophages with a phenotype similar to human alveolar macrophages.
Databáze: OpenAIRE
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