Cyclothialidine, a novel DNA gyrase inhibitor. II. Isolation, characterization and structure elucidation
| Autor: | Yoshiko Itezono, Nobuo Shimma, Naoki Nakada, Tatsuo Ohtsuka, Tsutomu Kamiyama, Kazuteru Yokose, Noboru Nakayama, Junko Watanabe |
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| Rok vydání: | 1994 |
| Předmět: |
Magnetic Resonance Spectroscopy
Molecular Sequence Data Amberlite DNA gyrase Peptides Cyclic Column chromatography Heterocyclic Compounds Drug Discovery Escherichia coli Topoisomerase II Inhibitors Pyrroles Amino Acid Sequence Amino Acids Chromatography High Pressure Liquid Streptomyces filipinensis Antibacterial agent Pharmacology chemistry.chemical_classification Chromatography biology Streptomycetaceae biology.organism_classification Streptomyces Enzyme chemistry Actinomycetales |
| Zdroj: | The Journal of antibiotics. 47(1) |
| ISSN: | 0021-8820 |
| Popis: | Cyclothialidine is a novel DNA gyrase inhibitor produced by Streptomyces filipinensis NR 0484. It was isolated from the culture broth by charcoal adsorption, Diaion HP-21, Amberlite CG-50, DEAE Toyopearl, and Toyopearl HW-40 SF column chromatography. The structure of cyclothialidine was determined to be a unique twelve membered lactone by amino acid analysis and various 2D-NMR experiments. Cyclothialidine inhibited Escherichia coli DNA gyrase with an IC50 of 30 ng/ml. |
| Databáze: | OpenAIRE |
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