Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeast
| Autor: | Hui Jin, Jinbo Fan, Bailey A. Koch, Hong-Guo Yu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Nuclear Envelope Health Toxicology and Mutagenesis LINC complex Protein domain Cell Cycle Proteins Plant Science macromolecular substances Saccharomyces cerevisiae Biochemistry Genetics and Molecular Biology (miscellaneous) Microtubules 03 medical and health sciences 0302 clinical medicine Heterotrimeric G protein Chromosome Segregation Inner membrane Nuclear Matrix Cytoskeleton Homologous Recombination Research Articles 030304 developmental biology 0303 health sciences Ecology Chemistry Membrane Proteins Nuclear Proteins Telomere Transmembrane protein Yeast Cell biology Meiosis Saccharomycetales SUN domain Homologous recombination 030217 neurology & neurosurgery Research Article |
| Zdroj: | Life Science Alliance |
| ISSN: | 2575-1077 |
| Popis: | The canonical LINC complex is composed of two different transmembrane proteins; this work reveals the heterotrimeric composition of the telomere-associated LINC complex in budding yeast. The linker of the nucleoskeleton and cytoskeleton (LINC) complex is composed of two transmembrane proteins: the KASH domain protein localized to the outer nuclear membrane and the SUN domain protein to the inner nuclear membrane. In budding yeast, the sole SUN domain protein, Mps3, is thought to pair with either Csm4 or Mps2, two KASH-like proteins, to form two separate LINC complexes. Here, we show that Mps2 mediates the interaction between Csm4 and Mps3 to form a heterotrimeric telomere-associated LINC (t-LINC) complex in budding yeast meiosis. Mps2 binds to Csm4 and Mps3, and all three are localized to the telomere. Telomeric localization of Csm4 depends on both Mps2 and Mps3; in contrast, Mps2’s localization depends on Mps3 but not Csm4. Mps2-mediated t-LINC complex regulates telomere movement and meiotic recombination. By ectopically expressing CSM4 in vegetative yeast cells, we reconstitute the heterotrimeric t-LINC complex and demonstrate its ability to tether telomeres. Our findings therefore reveal the heterotrimeric composition of the t-LINC complex in budding yeast and have implications for understanding variant LINC complex formation. |
| Databáze: | OpenAIRE |
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