PDMS-PMOXA-Nanoparticles Featuring a Cathepsin B-Triggered Release Mechanism
| Autor: | Janine Hussner, Isabell Seibert, Henriette E. Meyer zu Schwabedissen, Fabiola Porta, Daniel Ehrsam |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
cathepsin B
Nanoparticle Peptide 02 engineering and technology lcsh:Technology Cathepsin B Article Extracellular matrix 03 medical and health sciences paclitaxel cancer General Materials Science enzyme-triggered-release lcsh:Microscopy 030304 developmental biology lcsh:QC120-168.85 Cathepsin chemistry.chemical_classification PDMS-PMOXA 0303 health sciences lcsh:QH201-278.5 lcsh:T 021001 nanoscience & nanotechnology Controlled release Cysteine protease Enzyme ovarian cancer chemistry lcsh:TA1-2040 Biophysics lcsh:Descriptive and experimental mechanics nanoparticles lcsh:Electrical engineering. Electronics. Nuclear engineering 0210 nano-technology lcsh:Engineering (General). Civil engineering (General) lcsh:TK1-9971 |
| Zdroj: | Materials Materials, Vol 12, Iss 17, p 2836 (2019) Volume 12 Issue 17 |
| ISSN: | 1996-1944 |
| Popis: | Background: It was our intention to develop cathepsin B-sensitive nanoparticles for tumor-site-directed release. These nanoparticles should be able to release their payload as close to the tumor site with a decrease of off-target effects in mind. Cathepsin B, a lysosomal cysteine protease, is associated with premalignant lesions and invasive stages of cancer. Previous studies have shown cathepsin B in lysosomes and in the extracellular matrix. Therefore, this enzyme qualifies as a trigger for such an approach. Methods: Poly(dimethylsiloxane)-b-poly(methyloxazoline) (PDMS-PMOXA) nanoparticles loaded with paclitaxel were formed by a thin-film technique and standard coupling reactions were used for surface modifications. Despite the controlled release mechanism, the physical properties of the herein created nanoparticles were described. To characterize potential in vitro model systems, quantitative polymerase chain reaction and common bioanalytical methods were employed. Conclusions: Stable paclitaxel-loaded nanoparticles with cathepsin B digestible peptide were formed and tested on the ovarian cancer cell line OVCAR-3. These nanoparticles exerted a pharmacological effect on the tumor cells suggesting a release of the payload. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |