Kainic acid induces expression of caveolin-1 in activated microglia in rat brain
| Autor: | Osamu Yasuhara, Wakoto Matsuda, Shigeko Takeuchi, Ikuo Tooyama |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Kainic acid Histology Blotting Western Caveolin 1 Hippocampus Biology Pathology and Forensic Medicine chemistry.chemical_compound Western blot Caveolae Caveolin medicine Animals Rats Wistar Kainic Acid Microscopy Confocal Microglia medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Brain General Medicine Molecular biology Rats medicine.anatomical_structure nervous system Biochemistry chemistry Cerebral cortex Biomarkers |
| Zdroj: | Folia Histochemica et Cytobiologica. 51:25-30 |
| ISSN: | 1897-5631 0239-8508 |
| DOI: | 10.5603/fhc.2013.004 |
| Popis: | Caveolin-1, a major constituent of caveolae, has been implicated in endocytosis, signal transduction and cholesterol transport in a wide variety of cells. In the present study, the expression of caveolin-1 was examined by immunohistochemistry in rat brain with or without systemic injection of kainic acid (KA). Caveolin-1 immunoreactivity was observed in capillary walls in brains of control rats. From one to seven days after KA injection, caveolin-1 immunoreactivity appeared in activated microglia in the cerebral cortex, hippocampus and other brain regions. The strongest immunoreactivity of microglia was seen after 3 days after KA administration. The expression of caveolin-1 was confirmed by RT-PCR and Western blot analysis, respectively. The induction of caveolin-1 expression in microglia activated in response to kainic acid administration suggests its possible role in a modulation of inflammation. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 25–30) |
| Databáze: | OpenAIRE |
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