Response of aquatic protists to electric field exposure
| Autor: | Domagoj Đikić, Ivan Čolić, Dino Grozić, Marko Miliša, Ana Ostojić, Tvrtko Mandic |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Intracellular Space medicine.disease_cause Euglena Superoxide dismutase Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Electricity Malondialdehyde Botany medicine Radiology Nuclear Medicine and imaging Paramecium caudatum Hydrogen peroxide L-Lactate Dehydrogenase Radiological and Ultrasound Technology biology Superoxide Dismutase Superoxide Cell Membrane Croatia Paramecium ROS SOD plate capacitor Hydrogen Peroxide Catalase biology.organism_classification 030104 developmental biology chemistry 030220 oncology & carcinogenesis biology.protein Biophysics Lipid Peroxidation Oxidative stress |
| Zdroj: | International Journal of Radiation Biology. 93:818-830 |
| ISSN: | 1362-3095 0955-3002 |
| DOI: | 10.1080/09553002.2017.1321809 |
| Popis: | Purpose To test the effects of short-term exposure of aquatic organisms to electric field (EF) with negligible magnetic component. Materials and methods We built a plate capacitor that served as a source of EF of strengths that can be found in nature near transmission lines. We exposed two cultured protist species Euglena viridis and Paramecium caudatum to EFs for 24 hours and monitored their abundance, morphology, intracellular superoxide anion (by dihydroethidium [DHE]), hydrogen peroxide by (H2DCF) and lipid peroxidation (MDA) contents, catalase (CAT) and superoxide dismutase (SOD) activity. Results We found that even short-term exposure to low strength EF causes changes in population abundance, morphology and oxidative stress response in both species. As the EF strength increased, abundance of both species decreased. However, at weaker EFs, fission rates were seemingly promoted. We noted a decrease in size in both organisms in directions perpendicular to their fission planes correlated with EF strength. DHE and H2DCF fluorescence intensity and SOD activity were higher in organisms exposed to the stronger EFs. Conclusions We suggest that the electric component of the field, rather than the magnetic, is the main cause of all the noted effects. As a result, aquatic organisms should be given greater importance in studies assessing the effects of EMFs in spite of the attenuating effects of water to EF strengths. |
| Databáze: | OpenAIRE |
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