Sulphur and biothiol metabolism determine toxicity responses and fate of mercury in Arabidopsis
| Autor: | Juan Sobrino-Plata, Cesar González-Berrocal, Victor Ortega-Campayo, Sandra Carrasco-Gil, Javier Abadía, María Muñoz-Pinilla, Carlos Conesa-Quintana, Luis E. Hernández, Cristina Ortega-Villasante, Ángel Barón-Sola, Ana Álvarez-Fernández |
|---|---|
| Přispěvatelé: | Universidad Autonoma de Madrid (UAM), Universidad Politécnica de Madrid (UPM), Aula Dei Experimental Station (CSIC), Work supported by the Spanish State Research Agency (AEI) cofinanced with the European Regional Development Fund (ERDF) (projects AGL2014-53771-R and AGL2017-87591-R to LEH and AGL201675226-R to AAF and JA, AEI/ERDF, UE)., Agencia Estatal de Investigación (España), European Commission, Abadía Bayona, Javier [0000-0001-5470-5901], Álvarez-Fernández, Ana [0000-0003-4568-1201], Abadía Bayona, Javier, Álvarez-Fernández, Ana |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant mercury stress [SDV]Life Sciences [q-bio] Mutant Plant Science medicine.disease_cause 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Biosynthesis Arabidopsis medicine Ecology Evolution Behavior and Systematics ATP synthase biology Biothiol dynamics Xylem Plant S-assimilation Metabolism Glutathione biology.organism_classification 3. Good health 030104 developmental biology chemistry Biochemistry 13. Climate action Xylem Hg-PC complexes biology.protein Agronomy and Crop Science Oxidative stress 010606 plant biology & botany |
| Zdroj: | Environmental and Experimental Botany Environmental and Experimental Botany, Elsevier, 2021, 182, pp.104302. ⟨10.1016/j.envexpbot.2020.104302⟩ Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0098-8472 |
| Popis: | 14 Pags.- 7 Figs. Mercury (Hg) is one of the most hazardous pollutants released by humans and is of global environmental concern. Mercury causes oxidative stress and strong cellular damages in plants, which can be attenuated by the biosynthesis of thiol-rich peptides (biothiols), including glutathione (GSH) and phytochelatins (PCs). We analysed Hg tolerance and speciation in five Arabidopsis thaliana genotypes, the wild-type Col-0, three knockdown γ-glutamylcysteine synthetase (γECS) mutants and a knockout PC synthase (PCS) mutant. Mercury-PC complexes were detected in roots by HPLC-ESI-TOFMS, with its abundance being limited in γECS mutants. Analysis of Hg-biothiol complexes in the xylem sap revealed that HgPC2 occurs in wild-type Col-0 Arabidopsis, suggesting that Hg could be translocated associated with thiol-rich metabolites. Twenty genes involved in sulphur assimilation, GSH and PCs synthesis were differentially expressed in roots and shoots, implying a complex regulation, possibly involving post-translational mechanisms independent of GSH cellular levels. In summary, the present study describes the importance of biothiol metabolism and adequate GSH levels in Hg tolerance and identifies for the first time Hg-PC complexes in the xylem sap. This finding supports the notion that Hg-biothiol complexes could contribute to Hg mobilisation within plants. Work supported by the Spanish State Research Agency (AEI) co-financed with the European Regional Development Fund (ERDF) (projects AGL2014-53771-R and AGL2017-87591-R to LEH and AGL2016-75226-R to AAF and JA; AEI/ERDF, UE). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect